Contaminant analysis in biota extracts can be hampered by matrix interferences caused by, for example, co-extracted lipids that compromise the quality of the analytical data and require frequent maintenance of the analytical instruments. In the present study, using gas chromatography coupled to high resolution mass spectrometry (GC-HRMS), we aimed to develop and validate a straightforward, robust, and reproducible cleanup method with acceptable recoveries for diverse compound classes with a wide range of physicochemical properties representative of pollutant screening in biota extracts. We compared Oasis PRiME HLB cartridges, Agilent Captiva EMR-Lipid cartridges, and “Freeze-Out” with salmon lipids spiked with 113 target chemicals. The EMR-Lipid cartridges provided extracts with low matrix effects at reproducible recoveries of the multi-class target analytes (93 ± 9% and 95 ± 7% for low and high lipid amounts, respectively). The EMR-Lipid cartridges were further tested with spiked pork lipids submitted to total extraction or silicone-based passive sampling. Reproducible recoveries were achieved and matrix residuals were largely removed as demonstrated gravimetrically for both types of extracts. Ion suppression of halogenated compounds was not as efficiently removed by the cleanup of total and silicone-based extracts of pork lipids as for the salmon lipids. However, the samples with clean up provided better instrument robustness than those without cleanup. Hence, EMR-Lipid cartridges were shown to be efficient as a cleanup method in multi-class monitoring of biota samples and open up new possibilities as a suitable cleanup method for silicone extracts in biota passive sampling studies using GC-HRMS analysis. Environ Toxicol Chem 2021;40:2693–2704. © 2021 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

中文翻译：

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从全萃取和基于有机硅的被动采样中去除生物群提取物中的干扰基质成分

生物群提取物中的污染物分析可能会受到基质干扰的阻碍，例如，共提取脂质会影响分析数据的质量并需要经常维护分析仪器。在本研究中，我们使用气相色谱与高分辨率质谱 (GC-HRMS) 结合，旨在开发和验证一种简单、稳健且可重现的净化方法，该方法对具有广泛物理化学性质的各种化合物类别具有可接受的回收率，具有代表性生物群提取物中的污染物筛选。我们将 Oasis PRiME HLB 小柱、Agilent Captiva EMR-Lipid 小柱和“Freeze-Out”与添加了 113 种目标化学品的鲑鱼脂质进行了比较。EMR-Lipid 小柱在多类目标分析物的可重复回收率（低和高脂质量分别为 93 ± 9% 和 95 ± 7%）下提供具有低基质效应的提取物。对 EMR-Lipid 墨盒进行了进一步测试，其中添加了猪肉脂质，并进行了全萃取或基于有机硅的被动采样。实现了可重现的回收率，并且基质残留物在很大程度上被去除，这两种提取物的重量分析都证明了这一点。通过净化猪肉脂质的总提取物和硅基提取物，卤代化合物的离子抑制效果不如鲑鱼脂质有效。然而，经过净化的样品比未经净化的样品提供了更好的仪器稳定性。因此，环境毒物化学2021；40:2693–2704。© 2021 作者。Wiley Periodicals LLC 代表 SETAC 出版的Environmental Toxicology and Chemistry 。