This Optimized Multicolor Immunofluorescence Panel was designed to identify and quantify all principal leukocyte populations in human blood using a minimum number of markers. We achieved this goal using a carefully selected combination of 14 surface markers compatible with standard flow cytometric instruments and accessible to a particularly large research community. Optimized for use in whole blood, this panel allows polymorphonuclear cell identification, supports live cell recovery, and is well-suited for absolute cell counting applications in the original in vivo volume. Panel performance and the separation of populations are high, and virtually no cells remain undefined after gating. Besides the identification of neutrophils, eosinophils, basophils, T cells, natural killer cells, B cells, plasma cells, monocytes, myeloid dendritic cells and plasmacytoid dendritic cells, this panel also covers progenitor cells and may therefore be attractive for stem cell researchers. Envisioned applications of this panel include immune monitoring within clinical trials, initial discovery to inform subset-targeted panels, and clinical diagnostics. In summary, this panel offers a broadly applicable platform for immune cell identification, quantification and characterization in human samples, particularly whole blood.

中文翻译：

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OMIP 077：使用广泛适用的 14 色面板定义所有主要人类白细胞群

这种优化的多色免疫荧光试剂盒旨在使用最少数量的标记物来识别和量化人体血液中的所有主要白细胞群。我们通过精心挑选的 14 种表面标志物组合实现了这一目标，这些标志物与标准流式细胞仪仪器兼容，并且可供特别大的研究团体使用。该面板针对全血进行了优化，可识别多形核细胞，支持活细胞回收，非常适合原始体内体积中的绝对细胞计数应用。面板性能和群体分离度很高，门控后几乎没有细胞保持未定义。除了中性粒细胞、嗜酸性粒细胞、嗜碱性粒细胞、T细胞、自然杀伤细胞、B细胞、浆细胞、单核细胞、髓树突细胞和浆细胞样树突细胞，该小组还涵盖了祖细胞，因此可能对干细胞研究人员具有吸引力。该小组的设想应用包括临床试验中的免疫监测、为亚组靶向小组提供信息的初步发现和临床诊断。总之，该面板为人体样本，特别是全血中的免疫细胞鉴定、量化和表征提供了一个广泛适用的平台。