Recently, environmental DNA (eDNA) metabarcoding techniques have been applied to biodiversity investigations in aquatic ecosystems. However, no study has yet tested whether this technique is effective for water bodies in which extensive algal blooms break out. In this study, fish eDNA metabarcoding was carried out in Lake Taihu, which experiences extensive algal blooms, to confirm whether the technique is also effective for fish diversity research in ecosystems with frequent and extensive blooms. In December 2016, three samples were collected, including one collected in the presence of algal blooms and two collected in the absence of algal blooms. In August 2017, six samples were collected, including three collected in the presence of algal blooms and three in the absence of algal blooms. Equal amount of water samples (1 L) was collected from each site; however, the actual amount of filtrate varied with the site. Twenty-seven freshwater fish species were detected from the water samples collected in Lake Taihu. The results showed that the composition of the detected species did not differ whether or not blooms were present. However, the amount of filtration could influence the number of species detected. The results suggest that future eDNA metabarcoding studies under similar water environments should increase the amount of filtration to maximize number of species detected.

最近，环境 DNA (eDNA) 元条形码技术已应用于水生生态系统的生物多样性调查。然而，还没有研究测试这种技术是否对爆发大规模藻华的水体有效。在这项研究中，在经历了大量藻华的太湖中进行了鱼类 eDNA 元条形码编码，以确认该技术是否也适用于频繁和广泛藻华的生态系统中的鱼类多样性研究。2016 年 12 月，采集了三个样本，其中一个是在藻华存在的情况下采集的，另外两个是在没有藻华的情况下采集的。2017 年 8 月，收集了 6 个样本，其中 3 个在有藻华的情况下采集，3 个在没有藻华的情况下采集。从每个地点收集等量的水样（1 L）；然而，实际的滤液量因场地而异。从太湖采集的水样中检测到27种淡水鱼。结果表明，无论是否存在水华，检测到的物种的组成没有差异。然而，过滤量可能会影响检测到的物种数量。结果表明，未来类似水环境下的 eDNA 元条形码研究应该增加过滤量，以最大限度地检测到的物种数量。结果表明，无论是否存在水华，检测到的物种的组成没有差异。然而，过滤量可能会影响检测到的物种数量。结果表明，未来类似水环境下的 eDNA 元条形码研究应该增加过滤量，以最大限度地检测到的物种数量。结果表明，无论是否存在水华，检测到的物种的组成没有差异。然而，过滤量可能会影响检测到的物种数量。结果表明，未来类似水环境下的 eDNA 元条形码研究应该增加过滤量，以最大限度地检测到的物种数量。