This study reports the characterization of a collection of 98 strains of L. garvieae using phenotypic, antigenic, genetic, proteomic, and chemotaxonomic methods, as well as the study of their virulence. Molecular (RAPD and REP-PCR) and proteomic (MALDI-TOF-MS) techniques allowed the separation of strains of L. garvieae isolated from human and bovine from those strains of L. garvieae isolated from the aquatic environment. On the other hand, PCR typing methods showed that the L. garvieae strains tested did not belong to serotype II and did not carry the capsule gene cluster described in the genome or plasmid of Japanese L. garvieae strains. The different clonal patterns observed in L. garvieae using RAPD and REP-PCR (7 profiles) and MALDI-TOF (six patterns) were closely related to the isolation source of L. garvieae strains but not with the virulence of the strains. In addition, two protein antigenic variants (W1 and W2), differing in reactivity in the range of 35 to 70 KDa, were detected using rabbit and rainbow trout antisera. Both antisera detected two highly immunogenic protein bands of 30 and 45 KDa. These results could contribute to a better understanding of the pathogenesis of lactococcosis and could help in the selection of targets for the development of effective vaccines.

本研究报告了使用表型、抗原、遗传、蛋白质组学和化学分类学方法对 98 种 L. garvieae菌株的集合进行表征，以及对其毒力的研究。分子（RAPD和REP-PCR）和蛋白质组（MALDI-TOF-MS）技术允许L.的菌株的分离丁目从人和牛分离出L的那些菌株丁目从水生环境隔离。另一方面，PCR 分型方法表明，所测试的 L. garvieae菌株不属于血清型 II，并且不携带日本 L. garvieae菌株基因组或质粒中描述的荚膜基因簇。在 L. garvieae 中观察到的不同克隆模式使用RAPD和REP-PCR（7个模式）和MALDI-TOF（6个模式）与L. garvieae菌株的分离来源密切相关，但与菌株的毒力无关。此外，使用兔和虹鳟鱼抗血清检测到两种蛋白质抗原变体（W1 和 W2），其反应性在 35 至 70 KDa 的范围内不同。两种抗血清都检测到两条 30 和 45 KDa 的高免疫原性蛋白条带。这些结果有助于更好地了解乳球菌病的发病机制，并有助于选择开发有效疫苗的靶点。