Polyaniline was electrochemically polymerized onto the platinum electrode modified with a mixture of Nafion and multiwalled carbon nanotube (PANI/MWCNT/Nf/Pt) to detect ammonium ion. The nanobiosensor (ASNase/PANI/MWCNT/Nf/Pt) was then prepared by immobilizing L-asparaginase (L-ASNase) on the PANI/MWCNT/Nf nanocomposite. The prepared nanobiosensor was used for the rapid and sensitive detection of serum concentration of the anticancer agent L-asparagine (L-Asp) during chemotherapy. The nanobiosensor has dynamic ranges of zero to 180 μM. The sensitivity of the nanobiosensor was 0.829 μA μM⁻¹ cm⁻², and the response time was less than 30 s. The detection limit was 140 nM of L-Asp. The Michaelis–Menten constant () was measured to be 36.2 mM. The nanobiosensor was successfully applied for the determination of L-Asp in the blood samples of leukemia patients.

中文翻译：

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基于聚苯胺-多壁碳纳米复合材料的电流型酶生物传感器的开发，用于检测患者血清样本中的化疗药物

将聚苯胺电化学聚合到用 Nafion 和多壁碳纳米管 (PANI/MWCNT/Nf/Pt) 的混合物改性的铂电极上，以检测铵离子。然后通过将 L-天冬酰胺酶 (L-ASNase) 固定在 PANI/MWCNT/Nf 纳米复合材料上来制备纳米生物传感器 (ASNase/PANI/MWCNT/Nf/Pt)。制备的纳米生物传感器用于快速、灵敏地检测化疗期间抗癌剂L-天冬酰胺（L-Asp）的血清浓度。该纳米生物传感器的动态范围为0～180  μ M。纳米生物传感器的灵敏度为0.829  μ A  μ M ^-1  cm ^-2，响应时间小于30 s。L-Asp 的检测限为 140 nM。米氏常数 ()测量为 36.2 mM。该纳米生物传感器成功应用于白血病患者血样中L-Asp的测定。