Visible impairments in skin appearance, as well as a subtle decline in its functionality at the molecular level, are hallmarks of skin aging. Activation of the nuclear factor (erythroid-derived 2)-like 2 (Nrf2)-pathway, which is important in controlling inflammation and oxidative stress that occur during aging, can be triggered by sulforaphane (SFN), an isothiocyanate found in plants from the Brassicaceae family. This study aimed to assess the effects of SFN intake on age-related skin alterations. Male C57BL6 young (2 months) and old (21 months) mice were treated for 3 months with SFN diet (442.5 mg per kg) or control diet. The antioxidant capacities of the skin were increased in old SFN-treated animals as measured by mRNA levels of Nrf2 (P<.001) and its target genes NQO1 (P<.001) and HO1 (P<.01). Protein expression for Nrf2 was also increased in old SFN fed animals (P<.01), but not the protein expression of NQO1 or HO1. Additionally, ROS and MMP9 protein levels were significantly decreased (P<.05) in old SFN fed animals. Histopathological analysis confirmed that there was no difference in epidermal thickness in old, when compared to young, SFN treated animals, while the dermal layer thickness was lower in old vs. young, treated animals (P<.05). Moreover, collagen deposition was improved with SFN treatment in young (P<.05) and structurally significantly improved in the old mice (P<.001). SFN dietary supplementation therefore ameliorates skin aging through activation of the Nrf2-pathway.

皮肤外观的明显损伤以及分子水平上功能的微妙下降是皮肤衰老的标志。核因子（红细胞衍生 2）样 2 (Nrf2) 通路的激活对于控制衰老过程中发生的炎症和氧化应激非常重要，可以由萝卜硫素 (SFN) 触发，萝卜硫素是一种在植物中发现的异硫氰酸盐。十字花科。本研究旨在评估 SFN 摄入量对与年龄相关的皮肤变化的影响。雄性 C57BL6 年轻（2 个月）和年老（21 个月）小鼠用 SFN 饮食（442.5 mg/kg）或对照饮食治疗 3 个月。通过 Nrf2 ( P <.001) 及其靶基因 NQO1 ( P <.001) 和 HO1 ( P <.01) 的 mRNA 水平测量，经 SFN 治疗的年老动物的皮肤抗氧化能力有所增加。在年长 SFN 喂养的动物中，Nrf2 的蛋白表达也有所增加 ( P <.01)，但 NQO1 或 HO1 的蛋白表达没有增加。此外，在 SFN 喂养的老动物中，ROS 和 MMP9 蛋白水平显着降低 ( P <.05)。组织病理学分析证实，与年轻的 SFN 治疗动物相比，老年动物的表皮厚度没有差异，而老年动物的真皮层厚度低于年轻的治疗动物 ( P <.05 )。此外，SFN 治疗使年轻小鼠的胶原蛋白沉积得到改善 ( P <.05)，并且老年小鼠的结构显着改善 ( P <.001)。因此，SFN 膳食补充剂可通过激活 Nrf2 通路来改善皮肤衰老。