We investigated the effects of egg white protein hydrolysates (EWH) on orotic acid (OA)-induced nonalcoholic fatty liver (NAFL) in rats. Effects of the egg white protein (EWP) and EWH were also compared. Four groups of male Sprague-Dawley rats were separately fed AIN-76-based diets, supplemented with 20% casein for control, or with 1% OA, together with either 20% casein (OA), 20% EWP, or 20% EWH, respectively, for 3 d (developing stage) and 14 d (developed stage). In both feeding periods, animals from the OA group showed higher accumulation hepatic triacylglycerol (TAG) compared with those from the control group. In the 14-d experiment, dietary EWP and EWH significantly reduced the hepatic TAG levels. Intake of EWP reduced liver fat in OA-fed rats by 61%, while EWH reduced it by 92%. In addition, EWH restored the OA-induced high serum-TAG level to that seen in the control group. The 3 d experiment showed that consumption of EWH improved the expression of hepatic MTP, that was reduced by OA, without changing Mttp gene expression. It also increased the hepatic synthesis of PC and PE by enhancing the transcription of Pcyt1 and Pemt genes. Inclusion of EWP and EWH in the diet improves the OA-induced NAFL. EWH reduces the liver TAG better than EWP, and works more rapidly. Dietary EWH ameliorates OA-induced NAFL by promoting the secretion of hepatic TAG.

我们研究了蛋清蛋白水解物 (EWH) 对乳清酸 (OA) 诱导的大鼠非酒精性脂肪肝 (NAFL) 的影响。还比较了蛋清蛋白 (EWP) 和 EWH 的效果。四组雄性 Sprague-Dawley 大鼠分别饲喂基于 AIN-76 的饮食，补充 20% 酪蛋白作为对照，或添加 1% OA，以及 20% 酪蛋白 (OA)、20% EWP 或 20% EWH ，分别为 3 d（发展阶段）和 14 d（发展阶段）。在两个喂养期间，与对照组相比，OA 组的动物表现出更高的肝脏三酰基甘油 (TAG) 积累。在 14 天的实验中，膳食 EWP 和 EWH 显着降低了肝脏 TAG 水平。摄入 EWP 可使 OA 喂养大鼠的肝脏脂肪减少 61%，而 EWH 减少 92%。此外，EWH 将 OA 诱导的高血清 TAG 水平恢复到对照组中的水平。3 d 实验表明，EWH 的消耗改善了肝脏 MTP 的表达，OA 降低了 MTP 的表达，但没有改变Mttp基因表达。它还通过增强Pcyt1和Pemt基因的转录来增加 PC 和 PE 的肝脏合成。在饮食中加入 EWP 和 EWH 可改善 OA 诱导的 NAFL。EWH 比 EWP 能更好地降低肝脏 TAG，并且起效更快。膳食 EWH 通过促进肝脏 TAG 的分泌来改善 OA 诱导的 NAFL。