Background: Tylophora hirsuta (Wall) has long been used as traditional medicine for the treatment of diabetes. The current study is designed to evaluate the anti-diabetic and anti-inflammatory activity of different extracts of aerial parts of Tylophora hirsuta.

Methods: Sequential maceration was conducted to obtain extracts. Total phenolic contents were determined by the Folin-Ciocalteau method. The anti-oxidant activity was assessed by DPPH free radical scavenging assay. The extracts were tested for its inhibitory activity against α-amylase in-vitro. In-vivo anti-diabetic assay was conducted using alloxan-induced diabetic model and OGTT was conducted on normal rats. ELISA was used to determine the pro-inflammatory cytokines (TNF-α and IL-6). The polyphenolic composition of the extract was analyzed using an HPLC system.

Results: Aqueous extract exhibited highest total phenolic contents (985.24± 3.82 mg GAE/100 g DW), antioxidant activity (IC50 = 786.70 ± 5.23 μg/mL), and alpha-amylase inhibition (IC50 =352.8 μg/mL). The aqueous extract of Tylophora hirsuta showed remarkable in-vivo anti-diabetic activity. Results were compared with standard drug glibenclamide. Alloxan induced diabetic mediated alterations in liver function enzymes, renal function determinants, and lipid parameters were significantly restored in aqueous extract treated diabetic rats. A significant reduction in pro-inflammatory cytokines (p<0.001) was observed when compared to the control group. HPLC analysis confirms the presence of quercetin, gallic acid, cinnamic acid, and p-coumaric acid.

Conclusion: These results showed that Tylophora hirsuta possesses strong anti-diabetic and anti-inflammatory potentials and justify its folklore use for the management of diabetes.

背景：Tylophora hirsuta (Wall) 长期以来一直被用作治疗糖尿病的传统药物。目前的研究旨在评估 Tylophora hirsuta 地上部分不同提取物的抗糖尿病和抗炎活性。

方法：进行顺序浸渍以获得提取物。总酚含量通过 Folin-Ciocalteau 方法测定。通过DPPH自由基清除试验评估抗氧化活性。体外测试提取物对α-淀粉酶的抑制活性。使用四氧嘧啶诱导的糖尿病模型进行体内抗糖尿病试验，并在正常大鼠上进行 OGTT。ELISA 用于确定促炎细胞因子（TNF-α 和 IL-6）。使用 HPLC 系统分析提取物的多酚组成。

结果：水提物表现出最高的总酚含量 (985.24± 3.82 mg GAE/100 g DW)、抗氧化活性 (IC50 = 786.70 ± 5.23 μg/mL) 和 α-淀粉酶抑制 (IC50 =352.8 μg/mL)。Tylophora hirsuta 的水提取物显示出显着的体内抗糖尿病活性。结果与标准药物格列本脲进行比较。在水提取物治疗的糖尿病大鼠中，四氧嘧啶诱导的糖尿病介导的肝功能酶、肾功能决定因素和脂质参数的改变显着恢复。与对照组相比，观察到促炎细胞因子显着减少（p<0.001）。HPLC 分析证实了槲皮素、没食子酸、肉桂酸和对香豆酸的存在。

结论：这些结果表明，Tylophora hirsuta 具有强大的抗糖尿病和抗炎潜力，并证明其在民间传说中用于治疗糖尿病是合理的。