The growth, development and reproduction of Eriocheir sinensis are influenced by salinity changes. In order to study the response of juvenile Chinese mitten crab to salinity, the crabs were exposed to 5 salinity conditions (0, 3, 6, 12 and 18‰) for 48 h. The calcium content was detected in the hepatopancreas, posterior gill, intestine, haemolymph, antennal gland and carapace. The hepatopancreas, posterior gills and intestines were selected for subsequent experiments, and their histopathological changes and calreticulin (CRT) gene expression levels were evaluated. The calcium content of hepatopancreas and intestine was highest at 6‰ salinity and that of gill at 3‰ salinity. With the increase in salinity, the degree of damage was more obvious in tissues. Compared with the control group (0‰), CRT expression in the gills decreased significantly. However, in the hepatopancreas and intestine, CRT expression increased at 3‰ salinity and decreased at 3–6‰ salinity. Overall, our results showed that juvenile E. sinensis could survive short-term exposure to saline water within a certain salinity range (<18‰). However, a high concentration of saline water exposed the juvenile crab to excessive stress, resulting in serious tissue damage, which was not conducive to the long-term survival of juvenile crabs.

中文翻译：

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不同盐度条件下中华绒螯蟹幼蟹钙含量、组织病理学及钙网蛋白表达的变化

盐度变化影响中华绒螯蟹的生长、发育和繁殖。为了研究中华绒螯蟹幼蟹对盐度的响应，将蟹类暴露于5个盐度条件（0、3、6、12和18‰）48小时。在肝胰腺、后鳃、肠、血淋巴、触角腺和甲壳中检测到钙含量。选择肝胰腺、后鳃和肠进行后续实验，及其组织病理学变化和钙网蛋白（CRT）) 基因表达水平进行了评估。6‰盐度时肝胰腺和小肠钙含量最高，3‰盐度时鳃钙含量最高。随着盐度的增加，组织中的损伤程度更加明显。与对照组（0‰）相比，鳃中CRT表达显着降低。然而，在肝胰腺和肠道中，CRT表达在 3‰ 盐度下增加，在 3-6‰ 盐度下下降。总体而言，我们的结果表明，幼鱼E . 中华短期暴露于一定盐度范围内（<18‰）的盐水中可以存活。然而，高浓度的盐水使幼蟹承受过大的压力，造成严重的组织损伤，不利于幼蟹的长期生存。