Abstract

Photoluminescence spectroscopy experiments have been performed to study the interaction of Nafion polymer membrane with protein particles upon swelling in a saline solution based on ordinary (natural) water or on deuterium-depleted water (DDW). This study was motivated by the fact that Nafion swelling in natural water is accompanied by efficient “unwinding” of polymer fibers into the liquid bulk, whereas no unwinding was found in DDW. In addition, the set of polymer fibers unwound into the liquid bulk is similar to the extracellular matrix (glycocalix) on the cellular membrane surface. It is of interest to refine the role of unwound fibers in the interaction of protein macromolecules with the polymer membrane surface. The interaction of protein macromolecules with the membrane surface was found to induce luminescence quenching/enhancement effects, specific for different proteins. In other words, different dynamic modes arise in the dependence of luminescence intensity from Nafion surface on the soaking time.

中文翻译：

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水性蛋白质悬浮液中聚合物膜的溶胀：光致发光光谱实验

摘要

已经进行了光致发光光谱实验来研究 Nafion 聚合物膜与蛋白质颗粒在基于普通（天然）水或贫氘水 (DDW) 的盐水溶液中膨胀时的相互作用。这项研究的动机是，天然水中的 Nafion 膨胀伴随着聚合物纤维有效地“解绕”到液体本体中，而在 DDW 中没有发现解绕。此外，展开到液体本体中的一组聚合物纤维类似于细胞膜表面上的细胞外基质（糖杯）。细化未缠绕纤维在蛋白质大分子与聚合物膜表面相互作用中的作用是很有趣的。发现蛋白质大分子与膜表面的相互作用诱导发光淬灭/增强效应，针对不同的蛋白质。换句话说，Nafion 表面的发光强度对浸泡时间的依赖性会产生不同的动态模式。