Immunological stress decreases feed intake, suppresses growth and induces economic losses. However, the underlying molecular mechanism remains unclear. Label-free liquid chromatography and mass spectrometry (LC-MS) proteomics techniques were employed to investigate effects of immune stress on the hepatic proteome changes of Arbor Acres broilers (Gallus Gallus domesticus) challenged with Escherichia coli lipopolysaccharide (LPS). Proteomic analysis indicated that 111 proteins were differentially expressed in the liver of broiler chickens from the immune stress group. Of these, 28 proteins were down-regulated, and 83 proteins were up-regulated in the immune stress group. Enrichment analysis showed that immune stress upregulated the expression of hepatic proteins involved in defense function, amino acid catabolism, ion transport, wound healing, and hormone secretion. Furthermore, immune stress increased valine, leucine and isoleucine degradation pathways. The data suggests that growth depression of broiler chickens induced by immune stress is triggered by hepatic proteome alterations, and provides a new insight into the mechanism by which immune challenge impairs poultry production.

中文翻译：

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肉鸡（生长抑制的分子机制鸡内金免疫应激介导）：肝脏蛋白质组研究

免疫应激会降低采食量、抑制生长并导致经济损失。然而，潜在的分子机制仍不清楚。无标记液相色谱和质谱 (LC-MS) 蛋白质组学技术被用来研究免疫应激对大肠埃希氏菌脂多糖 (LPS) 攻击的 Arbor Acres 肉鸡 (Gallus Gallusdomesus) 肝脏蛋白质组变化的影响。蛋白质组学分析表明，免疫应激组肉鸡肝脏中有111种蛋白质差异表达。其中，28种蛋白质在免疫应激组中下调，83种蛋白质上调。富集分析表明，免疫应激上调了参与防御功能、氨基酸分解代谢、离子转运、伤口愈合、和激素分泌。此外，免疫应激增加了缬氨酸、亮氨酸和异亮氨酸的降解途径。数据表明免疫应激引起的肉鸡生长抑制是由肝脏蛋白质组改变引发的，并为免疫挑战损害家禽生产的机制提供了新的见解。