Natural alleles that control multiple disease resistance (MDR) are valuable for crop breeding. However, only one MDR gene has been cloned in maize, and the molecular mechanisms of MDR remain unclear in maize. In this study, through map-based cloning we cloned a teosinte-derived allele of a resistance gene, Mexicana lesion mimic 1 (ZmMM1), which causes a lesion mimic phenotype and confers resistance to northern leaf blight (NLB), gray leaf spot (GLS), and southern corn rust (SCR) in maize. Strong MDR conferred by the teosinte allele is linked with polymorphisms in the 3′ untranslated region of ZmMM1 that cause increased accumulation of ZmMM1 protein. ZmMM1 acts as a transcription repressor and negatively regulates the transcription of specific target genes, including ZmMM1-target gene 3 (ZmMT3), which functions as a negative regulator of plant immunity and associated cell death. The successful isolation of the ZmMM1 resistance gene will help not only in developing broad-spectrum and durable disease resistance but also in understanding the molecular mechanisms underlying MDR.

控制多重抗病性（MDR）的天然等位基因对作物育种很有价值。然而，在玉米中仅克隆了一个MDR基因，并且在玉米中MDR的分子机制尚不清楚。在这项研究中，通过基于图谱的克隆，我们克隆了一个抗性基因的类蜀黍衍生等位基因，墨西哥病斑模拟 1 ( ZmMM1 )，它引起病斑模拟表型并赋予对北方叶枯病 (NLB)、灰叶斑病 ( GLS) 和玉米中的南方玉米锈病 (SCR)。teosinte 等位基因赋予的强 MDR 与ZmMM1 3' 非翻译区的多态性有关导致ZmmM1蛋白积累增加。ZmmM1 充当转录抑制因子并负调节特定靶基因的转录，包括ZmMM1 靶基因 3 ( ZmMT3 )，其作为植物免疫和相关细胞死亡的负调节因子。ZmmM1抗性基因的成功分离不仅有助于开发广谱和持久的抗病性，而且有助于了解MDR的分子机制。