Gastric cancer is considered a major cause of cancer-associated death, worldwide. Conjugation of thiosemicarbazones with metal nanoparticles (NPs) has been reported to enhance their anti-proliferative effect against cancer cells. This work aimed to synthesize NiO NPs, functionalize them with glutamic acid, and conjugate them with thiosemicarbazide (TSC). Characterization of the NiO@Glu-TSC was performed using Fourier-transform infrared (FT-IR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-Ray diffraction (XRD), energy dispersive X-Ray (EDX), Dynamic light scattering (DLS), and zeta potential analysis. Cytotoxicity of the NiO@Glu-TSC NPs toward AGS cells and normal fibroblast cells was investigated using the MTT assay. Apoptosis induction in AGS cells was evaluated using Flow cytometry and Hoechst 33,258 staining assays. The FT-IR results showed the proper binding of TSC to NiO@Glu NPs. The crystallographic structure of the NPs was confirmed based on the XRD pattern. The EDX analysis confirmed the presence of Ni, O, C, N, and S elements. The Hydrodynamic size and zeta potential of the prepared NPs were 256 nm and − 34.2 mv, respectively. The spherical NiO@Glu/TSC NPs were synthesized with an average size of 35 nm in diameter. The cytotoxicity assay showed significantly higher toxicity of the NPs for AGS cells than normal fibroblast cells with IC₅₀ values of 220 and 390 µg/mL, respectively. Cell apoptosis induction was observed among AGS cells treated with the NPs. The NPs treated cells showed late apoptosis with nuclear fragmentation and clumping. This work revealed the efficient anti-proliferative potential of NiO@Glu/TSC cells, which could be used against gastric cancer after further characterization.

胃癌被认为是全球癌症相关死亡的主要原因。据报道，缩氨基硫脲与金属纳米粒子 (NPs) 的结合可增强它们对癌细胞的抗增殖作用。这项工作旨在合成 NiO NPs，用谷氨酸对其进行功能化，并将它们与氨基硫脲 (TSC) 结合。NiO@Glu-TSC 的表征使用傅里叶变换红外 (FT-IR)、扫描电子显微镜 (SEM)、透射电子显微镜 (TEM)、X 射线衍射 (XRD)、能量色散 X 射线 (EDX) )、动态光散射 (DLS) 和 zeta 电位分析。使用 MTT 测定研究了 NiO@Glu-TSC NPs 对 AGS 细胞和正常成纤维细胞的细胞毒性。使用流式细胞术和 Hoechst 33 评估 AGS 细胞的凋亡诱导，258 次染色检测。FT-IR 结果表明 TSC 与 NiO@Glu NPs 正确结合。基于XRD图谱确认了NPs的晶体结构。EDX 分析证实了 Ni、O、C、N 和 S 元素的存在。制备的 NPs 的流体动力学尺寸和 zeta 电位分别为 256 nm 和 - 34.2 mv。合成的球形 NiO@Glu/TSC NPs 平均直径为 35 nm。细胞毒性试验表明，NPs 对 AGS 细胞的毒性显着高于具有 IC 的正常成纤维细胞 制备的 NPs 的流体动力学尺寸和 zeta 电位分别为 256 nm 和 - 34.2 mv。合成的球形 NiO@Glu/TSC NPs 平均直径为 35 nm。细胞毒性试验表明，NPs 对 AGS 细胞的毒性显着高于具有 IC 的正常成纤维细胞 制备的 NPs 的流体动力学尺寸和 zeta 电位分别为 256 nm 和 - 34.2 mv。合成的球形 NiO@Glu/TSC NPs 平均直径为 35 nm。细胞毒性试验表明，NPs 对 AGS 细胞的毒性显着高于具有 IC 的正常成纤维细胞_{50 个}值分别为 220 和 390 µg/mL。在用NPs处理的AGS细胞中观察到细胞凋亡诱导。NPs 处理的细胞显示出晚期细胞凋亡，并伴有核碎裂和结块。这项工作揭示了 NiO@Glu/TSC 细胞的高效抗增殖潜力，在进一步表征后可用于抗胃癌。