Introduction: Physical exercise is an important component of managing Alzheimer’s disease (AD). miRNAs can be modulated by exercise intervention. Objective: The study explored the involvement and potential mechanism of miR-192-5p in the protective effect of physical exercise on AD. Methods: Ninety AD patients were enrolled, in which 45 cases accepted cycling training for continuous 3 months. The expression changes of miR-192-5p before and after exercise were analyzed by reverse transcription-quantitative PCR. 8-month-old APP/PS1 double Tg mice were used as the AD animal model. Mice in the voluntary exercise (VE) group received VE for 4 weeks. Morris water maze (MWM) test was used to evaluate the learning and memory function. Enzyme-linked immunosorbent assay was used to calculate the level of IL-1β, IL-6, and TNF-α. Results: AD patients showed elevated MMSE scores, decreased ADAS-cog and NPI-Q scores after 3 months of exercise. miR-192-5p was downregulated in the serum of AD patients and correlated with the levels of MMSE score, ADAS-cog, and NPI-Q score. A positive association was detected between serum miR-192-5p with TNF-α, IL-6, and IL-1β levels. MiR-192-5p is downregulated in the hippocampus tissues of mice after VE. Overexpression of miR-192-5p reversed the neuroprotective effect of exercise on AD in mice and promoted the inflammatory response of AD mice. Conclusion: MiR-192-5p can be modulated by the exercise intervention and involved in the protective effect of exercise on AD.
Neuroimmunomodulation

中文翻译：

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运动介导的 miR-192-5p 改变与阿尔茨海默病的认知改善有关

简介：体育锻炼是管理阿尔茨海默病 (AD) 的重要组成部分。miRNA可以通过运动干预来调节。目的：本研究探讨了miR-192-5p在体育锻炼对AD保护作用中的作用及其潜在机制。方法：90 例 AD 患者入组，其中 45 例接受了连续 3 个月的自行车训练。通过逆转录-定量PCR分析运动前后miR-192-5p的表达变化。以8月龄APP/PS1双Tg小鼠为AD动物模型。自愿运动 (VE) 组的小鼠接受 VE 4 周。莫里斯水迷宫（MWM）测试用于评估学习和记忆功能。酶联免疫吸附试验用于计算IL-1β、IL-6和TNF-α的水平。结果：AD 患者在运动 3 个月后表现出 MMSE 评分升高，ADAS-cog 和 NPI-Q 评分降低。miR-192-5p 在 AD 患者血清中下调，并与 MMSE 评分、ADAS-cog 和 NPI-Q 评分水平相关。在血清 miR-192-5p 与 TNF-α、IL-6 和 IL-1β 水平之间检测到正相关。VE 后，MiR-192-5p 在小鼠海马组织中下调。miR-192-5p的过表达逆转了运动对AD小鼠的神经保护作用，促进了AD小鼠的炎症反应。结论： MiR-192-5p可通过运动干预进行调节，参与运动对AD的保护作用。
神经免疫调节