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Dietary Risk of blaESBL Producing Multidrug Resistant Enterobacteriaceae and their Inhibition by Artemisia herba-alba and Thymus algeriensis Essential Oils
Journal of Essential Oil Bearing Plants ( IF 2.1 ) Pub Date : 2021-07-12 , DOI: 10.1080/0972060x.2021.1937334
Mechaala Sara 1, 2 , Bouatrous Yamina 1, 2 , Erenler Ramazan 3 , Gok Mesut 4 , Adouane Selma 2, 5
Affiliation  

Abstract

The main objective of this study is to highlight the dietary risk of the prevalence of blaESBL producing multidrug-resistant Enterobacteriaceae in raw milk, besides evaluating the effect of Artemesia herba-alba and Thymus algeriensis essential oils against blaESBL producing Enterobacteriaceae isolates from raw milk. Isolation and molecular identification of ESBL producing Enterobacteriaceae and blaTEM, blaSHV, blaCTX-M genes encoding resistance were investigated by PCR and sequencing. The chemical composition of oils was effectuated by GC/MS. Antibacterial activity was tested by disc diffusion and microdilution methods. Six ceftazidime-resistant Enterobacteriaceae were identified; four of them were confirmed as ESBL producers and were found to be multi-drug resistant. blaSHV and blaTEM were found to be having the most dominant gene encoding resistance. The results of GC/MS analysis bestowed 31 and 35 compounds for A. herba-alba and T. algeriensis respectively. Klebsiella pneumonia SB6 (17.04 mm for A. herba-alba and 16.22 mm for T. algeriensis) revealed the highest zones of inhibition. MIC values were 1.56-12.5 mg/mL for both essential oils, while the MBC values were 6.25-25 mg/mL (A. herba-alba) and 12.5-25 mg/mL (T. algeriensis).



中文翻译:

blaESBL 产生多药耐药肠杆菌科细菌的饮食风险及其对白蒿和百里香精油的抑制作用

摘要

本研究的主要目的是强调生奶中产bla ESBL 的多重耐药肠杆菌科细菌流行的饮食风险,此外还评估了白蒿阿尔及利亚百里香精油对生奶中产bla ESBL肠杆菌科细菌的影响. 产ESBL肠杆菌bla TEM , bla SHV , bla CTX-M的分离及分子鉴定通过PCR和测序研究了编码抗性的基因。油的化学成分由 GC/MS 确定。通过圆盘扩散和微量稀释方法测试抗菌活性。鉴定出6株耐头孢他啶的肠杆菌;其中四人被确认为 ESBL 生产者,并被发现具有多重耐药性。bla SHVbla TEM被发现具有最主要的编码抗性基因。GC/MS 分析结果分别为A. herba-albaT. algeriensis提供了 31 和 35 个化合物。肺炎克雷伯菌SB6(A. herba-alba为 17.04 mm,A. herba-alba为 16.22 mmT. algeriensis ) 显示出最高的抑制区域。两种精油的 MIC 值为 1.56-12.5 mg/mL,而 MBC 值为 6.25-25 mg/mL(A. herba-alba)和 12.5-25 mg/mL(T. algeriensis)。

更新日期:2021-07-21
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