Background. Different common gene variants were related to coronary artery disease (CAD) in many studies. Yet, the relation of these loci to the severity of CAD is not completely elucidated. Methods. We enrolled 520 subjects (315 CAD cases and 205 controls). CAD presence and extension were assessed by coronary angiography (CAG). Genotyping of five SNPs (namely, rs2230806 (1051G > A) in ABCA1 on chromosome 9, rs2075291 (553G > T) in ApoA5 on chromosome 11, rs320 in LPL on chromosome 8 intron (T → G at position 481), rs10757278 (c.22114477A > G), and rs2383206 (c.22115026 A > G) on chromosome 9p21 locus) was performed by allele-specific PCR. The degree and site of arterial lesions were used to classify patients, tested for association with CAD severity, and related to allele dosage. Results. The polymorphisms rs2383206 and rs10757278 showed significant associations with 2- and 3-vessel coronary disease (p =0.003 and 0.006, respectively). The homozygous GG genotypes of rs10757278 was associated with higher frequency of left anterior descending (LAD), right coronary artery (RCA) and left circumflex (LCX) diseases (p =0.002, 0.016 and 0.002, respectively). The GG genotypes of rs2383206 were found in higher percentage in patients with left main (LM) trunk and left circumflex (LCX) diseases ( and 0.002, respectively). Conclusion. SNPs rs10757278 and rs2383206 allele dosage could predict CAD severity in the Saudi Arab population.

中文翻译：

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冠状动脉疾病：5 个位点与疾病严重程度的血管造影指数的关联研究

背景。在许多研究中，不同的常见基因变异与冠状动脉疾病 (CAD) 有关。然而，这些基因座与 CAD 严重程度的关系尚未完全阐明。方法。我们招募了 520 名受试者（315 名 CAD 病例和 205 名对照）。通过冠状动脉造影 (CAG) 评估 CAD 的存在和扩展。五个 SNP 的基因分型（即 9 号染色体上 ABCA1 中的 rs2230806（1051G > A），11 号染色体上 ApoA5 中的 rs2075291（553G > T），8 号染色体内含子上 LPL 中的 rs320（T → G 在位置 481），rs10757278（c .22114477A > G) 和 rs2383206 (c.22115026 A > G) 在染色体 9p21 基因座上）通过等位基因特异性 PCR 进行。动脉病变的程度和部位用于对患者进行分类，测试与 CAD 严重程度的关联，并与等位基因剂量相关。结果。多态性 rs2383206 和 rs10757278 与 2 支和 3 支血管冠状动脉疾病显着相关（分别为 p = 0.003 和 0.006）。rs10757278 的纯合 GG 基因型与左前降支 (LAD)、右冠状动脉 (RCA) 和左回旋支 (LCX) 疾病的较高频率相关（分别为 p = 0.002、0.016 和 0.002）。rs2383206的GG基因型在左主干（LM）和左旋支（LCX）疾病患者中的比例较高（和 0.002，分别）。结论。SNP rs10757278 和 rs2383206 等位基因剂量可以预测沙特阿拉伯人群的 CAD 严重程度。