当前位置: X-MOL 学术Conserv. Genet. Resour. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Development and characterization of SNP markers in many-lined sun skink (Eutropis multifasciata) from transcriptomic sequences
Conservation Genetics Resources ( IF 0.9 ) Pub Date : 2021-07-13 , DOI: 10.1007/s12686-021-01224-5
Youfu Lin 1 , Lian Chen 1 , Zijin Qian 2 , Hong Li 2 , Shiyu Shen 3 , Yicheng Huang 3 , Yu Du 4
Affiliation  

Many-lined sun skink (Eutropis multifasciata) is a medium sized viviparous scincid lizard. Insufficient molecular markers have limited the effective conservation and management of E. multifasciata. In the present study, 28 novel single nucleotide polymorphism (SNP) markers were developed from the transcriptome dataset of E. multifasciata using SNaPshot method. The minor allele frequency ranged from 0.117 to 0.483. The observed heterozygosity and expected heterozygosity ranged from 0.167 to 0.567 and from 0.210 to 0.508, respectively. Polymorphic information content ranged from 0.185 to 0.375. None of the loci deviated significantly from Hardy–Weinberg equilibrium and no significant linkage disequilibrium was found. Those polymorphic SNP markers will be helpful for the further studies on population structure and conservation genetics in E. multifasciata.



中文翻译:

从转录组序列开发和表征多线太阳石龙子 (Eutropis multifasciata) 中的 SNP 标记

多线太阳石龙子 ( Eutropis multifasciata ) 是一种中等大小的胎生 scincid 蜥蜴。分子标记不足限制了E. multifasciata的有效保护和管理。在本研究中,从E. multifasciata的转录组数据集开发了 28 个新的单核苷酸多态性 (SNP) 标记使用 SNaPshot 方法。次要等位基因频率范围为 0.117 至 0.483。观察到的杂合度和预期的杂合度分别介于 0.167 到 0.567 和 0.210 到 0.508 之间。多态信息含量从 0.185 到 0.375 不等。没有一个位点显着偏离 Hardy-Weinberg 平衡,也没有发现显着的连锁不平衡。这些多态性SNP标记将有助于进一步研究E. multifasciata种群结构和保护遗传学

更新日期:2021-07-13
down
wechat
bug