ABSTRACT

Xylogen-like proteins (XYLPs) are essential for plant growth, development, and stress responses. However, little is known about the XYLP gene family in grape and its protective effects against gray mold a destructive disease caused by Botrytis cinerea. We identified and characterized six common XYLPs in the Vitis vinifera genome (VvXYLPs). VvXYLP expression pattern analyses with B. cinerea infection showed that VvXYLP02 was significantly up-regulated in the resistant genotype but down-regulated or only slightly up-regulated in the susceptible genotype. VvXYLP02 overexpression in Arabidopsis thaliana significantly increased resistance to B. cinerea, indicating that the candidate gene has functional importance. Furthermore, JA treatment significantly up-regulated VvXYLP02 expression in V. vinifera. JA-responsive genes were also up-regulated in VvXYLP02 overexpression lines in A. thaliana under B. cinerea inoculation. These findings suggest that VvXYLP02, which is induced by JA upon the pathogen infection, enhances JA dependent response to enforce plant resistance against gray mold disease.

摘要

类木素蛋白 (XYLP) 对植物生长、发育和应激反应至关重要。然而，关于葡萄中的XYLP基因家族及其对灰霉病的保护作用知之甚少，灰霉病是由灰霉病菌引起的破坏性疾病。我们鉴定并表征了葡萄基因组 (VvXYLPs) 中的六种常见 XYLP。用B. cinerea感染的 VvXYLP 表达模式分析表明，VvXYLP02在抗性基因型中显着上调，但在易感基因型中下调或仅略微上调。VvXYLP02在拟南芥中的过表达显着增加了对B. cinerea的抗性，表明候选基因具有功能重要性。此外，JA 处理显着上调V. vinifera中的VvXYLP02表达。在B. cinerea接种下的拟南芥中的VvXYLP02过表达系中，JA 反应基因也上调。这些发现表明，在病原体感染时由 JA 诱导的 VvXYLP02 增强了 JA 依赖性反应，以增强植物对灰霉病的抵抗力。