Cell sorting is important in many studies, from basic science, such as cell biology, to biomedical applications, such as cell engineering and regenerative medicine. We have developed a photoinduced in situ cell detachment system using gold nanoparticle (AuNP)-embedded collagen hydrogels as a spatiotemporal cell sorting system, which is an image-guided cell sorting system. Here, we prepared AuNP-containing collagen coatings as a photosensitive cell scaffold. Various cells cultured on the coating materials were detached by photoirradiation and subsequent medium injection. The monolayer type of AuNP/collagen coatings showed efficient cell detachment with cell damage and may be applied to remove undesired cells. For cell damage suppression, the bilayer-type AuNP/collagen coatings were designed and prepared. We demonstrated the sorting of visually changed transfected CHO-K1 cells and human-induced pluripotent stem cell-derived cardiomyocytes using our system, which is useful for genome editing and regenerative medicine, respectively.

细胞分选在许多研究中都很重要，从基础科学（如细胞生物学）到生物医学应用（如细胞工程和再生医学）。我们开发了一种使用金纳米颗粒 (AuNP) 嵌入的胶原水凝胶作为时空细胞分选系统的光诱导原位细胞分离系统，这是一种图像引导的细胞分选系统。在这里，我们制备了含 AuNP 的胶原涂层作为光敏细胞支架。通过光照射和随后的培养基注射分离在涂层材料上培养的各种细胞。单层类型的 AuNP/胶原涂层显示出有效的细胞分离和细胞损伤，可用于去除不需要的细胞。为了抑制细胞损伤，设计并制备了双层型 AuNP/胶原蛋白涂层。