The purpose of this study was to determine the potentiality of the composting process and biological pretreatment of using P. florida to improve the nutritive value and enhance the digestibility of rice straw. A total of 40 rice straw samples with and without composting treated with P. florida at different substrate harvesting periods (full ramification, 1st, 2nd, and 3rd flushing) were evaluated by analyzing its chemical composition, in vitro digestibility, and scanning electron microscopy (SEM). Results showed that composted and uncomposted rice straw in the 2nd flush had the highest dry matter (DM) content of 96.07% and 95.56%, respectively. The highest crude protein (CP) content (10.57%) was obtained in composted rice straw in the 3rd flush. The composted rice straw treated with P. florida had low organic matter (OM) content but high concentrations of ash, cellulose, and nutrient digestibility. These results indicate that the composted rice straw in the 3rd flush was found to be the best in terms of sufficient degrading of lignin content, minimum loss in cellulose concentrations, enhanced crude protein content, and nutrient digestibility. Therefore, it is recommended to develop spent mushroom substrate feed supplements for possible use in ruminants.

本研究的目的是确定使用P. florida的堆肥过程和生物预处理在提高稻草营养价值和提高稻草消化率方面的潜力。通过分析其化学成分，在体外评估了在不同基质收获期（完全分枝、第 1、第 2 和第 3 次冲洗）处理的有和没有堆肥的共 40 个稻草样品。消化率和扫描电子显微镜 (SEM)。结果表明，第二次堆肥和未堆肥的稻草干物质（DM）含量最高，分别为 96.07% 和 95.56%。在第 3 次冲洗的堆肥稻草中获得了最高的粗蛋白 (CP) 含量 (10.57%)。用P. florida处理的堆肥稻草有机质 (OM) 含量低，但灰分、纤维素和养分消化率高。这些结果表明，在木质素含量充分降解、纤维素浓度损失最小、粗蛋白含量提高和养分消化率方面，发现第 3 次冲洗的堆肥稻草是最好的。因此，建议开发可能用于反刍动物的废弃蘑菇基质饲料添加剂。