Abstract

As an essential micronutrient, manganese (Mn) participates in diverse processes during plant growth and development. Excess accumulation of Mn in plants is toxic, and soybean (Glycine max) growth and production are severely limited by Mn toxicity. However, the molecular basis in adaptation to Mn toxicity for soybean remains largely unknown. In this study, RNA-seq analysis on soybean leaves was conducted, more than 44 million reads were generated, and a total of 38,022 expressed genes were identified. Compared to control, 2258 differentially expressed genes (DEGs), including 744 up-regulated and 1514 down-regulated ones, were obtained. Cellular process, cell part and binding function were the most enriched terms by GO analysis. Furthermore, 49 DEGs were identified in plant hormone signal transduction pathways by KEGG analysis. Among them, Mn toxicity up-regulated AHK, PIF, JAZ and TGA family DEGs might play important roles in the adaptation of soybean leaves to Mn toxicity.

Supplemental data for this article is available online at https://doi.org/10.1080/13102818.2021.1950566 .

摘要

作为一种必需的微量营养素，锰 (Mn) 参与植物生长和发育过程中的各种过程。锰在植物中的过量积累是有毒的，而大豆（甘氨酸最大) 生长和生产受到锰毒性的严重限制。然而，大豆适应锰毒性的分子基础在很大程度上仍然未知。在这项研究中，对大豆叶片进行了 RNA-seq 分析，产生了超过 4400 万个读数，共鉴定了 38022 个表达基因。与对照相比，获得了2258个差异表达基因（DEGs），包括744个上调基因和1514个下调基因。细胞过程、细胞部分和结合功能是 GO 分析中最丰富的术语。此外，通过KEGG分析在植物激素信号转导途径中鉴定出49个DEG。其中，Mn 毒性上调AHK、PIF、JAZ和TGA DEG 家族可能在大豆叶片对锰毒性的适应中起重要作用。

本文的补充数据可在 https://doi.org/10.1080/13102818.2021.1950566 在线获得。