Enteritis caused by CPV-2 antigenic variants (CPV-2a, 2b, and 2c) is frequently reported in dogs worldwide leading to significant morbidity and mortality. Here, we describe about a simple, single-step, ARMS-PCR strategy targeting the mutant 426 amino acid of VP2 to differentiate CPV-2 antigenic types. A total of 150 fecal samples were subjected to ARMS-PCR of which 18 were typed as CPV-2a, 79 were typed as CPV-2b, and 6 were typed as CPV-2c. The ARMS-PCR results were validated by randomly sequencing partial VP2 gene of 14 samples. Phylogenetic analysis of partial VP2 gene sequencing of each of the CPV-2 variants revealed that CPV-2a and CPV-2b isolates formed a separate clade of Indian lineage, while CPV-2c shared common evolutionary origin with Asian lineage. The developed technique is first of its kind, one-step, rapid, sequencing independent method for typing of CPV-2 antigenic variants.

由 CPV-2 抗原变体（CPV-2a、2b 和 2c）引起的肠炎在世界范围内的狗中经常报道，导致显着的发病率和死亡率。在这里，我们描述了一种简单的、单步的、ARMS-PCR 策略，该策略针对 VP2 的 426 个突变氨基酸来区分 CPV-2 抗原类型。总共150份粪便样本进行了ARMS-PCR，其中18份为CPV-2a，79份为CPV-2b，6份为CPV-2c。通过对14个样本的部分VP2基因进行随机测序来验证ARMS-PCR结果。部分VP2的系统发育分析每个 CPV-2 变体的基因测序显示 CPV-2a 和 CPV-2b 分离株形成了一个独立的印度谱系进化枝，而 CPV-2c 与亚洲谱系具有共同的进化起源。所开发的技术是同类中首创的、一步、快速、独立于测序的 CPV-2 抗原变体分型方法。