当前位置: X-MOL 学术Eur. J. Plant Pathol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Silver nanoparticles inactivate sclerotial formation in controlling white rot disease in onion and garlic caused by the soil borne fungus Stromatinia cepivora
European Journal of Plant Pathology ( IF 1.8 ) Pub Date : 2021-05-17 , DOI: 10.1007/s10658-021-02296-7
Osama M. Darwesh , Ibrahim E. Elshahawy

Under Egyptian conditions, the white rot disease, caused by the soil borne fungus Stromatinia cepivora, causes a major problem in the production of onion and garlic crops. The current study aims to control this disease using silver nanoparticles (AgNPs), biologically synthesized by Fusarium oxysporum. In in vitro assays, carried out on Potato Dextrose Agar (PDA) and Potato Dextrose Broth (PDB) media, the biosynthesized AgNPs at various concentrations i.e. 40, 80, 120, 160 and 200 mg/L, showed a great and promising antifungal activity against the linear growth, mycelial biomass and scelerotial germination of S. cepivora isolates. With increasing of AgNPs concentration the antifungal activity was increased and the application of 200 mg/L produced maximum antifungal activity. It was noted that AgNPs at all concentrations inactivate sclerotial formation on the treated culture media and to our knowledge; this observation is considered a first report. The protein profile of AgNPs-untreated fungal biomass showed the presence of band at 28 k Dalton. This band was absent in the profile of AgNPs-treated fungal biomass and may be related to the formation of sclerotia. In the potted experiment, the chemical control using of tebuconazole recorded the highest efficiency in reducing the incidence of white rot disease. Significant reduction in the incidence of white rot was obtained with AgNPs applied as transplants/clove dipping or stems base spraying or dipping plus spraying. In general, the combined treatment was more effective than these methods used individually. In field experiments, the double treatment, which included the treatment of dipping and spraying, led to a significant reduction in the disease incidence, whether in the field with a low or high level of sclerotia. Under low inoculum levels, significant control was achieved with all methods used. However, the method of dipping only did not give significant control of garlic white rot under high inoculum levels. Application of AgNPs provides an improvement in the growth and yield of bulbs for both onion and garlic plants grown under field conditions. Therefore, AgNPs can be used as nanofungicide against white rot disease and as nanofertilizers for onion and garlic productions.



中文翻译:

银纳米粒子灭活菌核形成以控制由土传真菌引起的洋葱和大蒜白腐病

在埃及条件下,由土传真Stromatinia cepivora引起的白腐病是洋葱和大蒜作物生产中的一个主要问题。目前的研究旨在使用由尖孢镰刀菌生物合成的银纳米颗粒 (AgNPs) 控制这种疾病。在马铃薯葡萄糖琼脂 (PDA) 和马铃薯葡萄糖肉汤 (PDB) 培养基上进行的体外测定中,不同浓度(即 40、80、120、160 和 200 mg/L)的生物合成 AgNPs 显示出强大且有前景的抗真菌活性反对S. cepivora的线性生长、菌丝体生物量和菌核萌发隔离。随着 AgNPs 浓度的增加,抗真菌活性增加,200 mg/L 的应用产生最大的抗真菌活性。据我们所知,所有浓度的 AgNPs 都会使处理过的培养基上的菌核形成失活。这一意见被视为第一次报告。AgNPs 未处理的真菌生物质的蛋白质谱显示在 28 k 道尔顿处存在条带。该条带在 AgNPs 处理的真菌生物质的轮廓中不存在,可能与菌核的形成有关。在盆栽试验中,使用戊唑醇的化学防治记录了降低白腐病发病率的最高效率。AgNPs 作为移植物/丁香浸渍或茎基喷洒或浸渍加喷洒获得了白腐病发生率的显着降低。一般来说,联合治疗比单独使用这些方法更有效。在田间试验中,无论是在菌核水平低还是高的田间,包括浸药和喷药在内的双重处理都显着降低了发病率。在低接种量下,使用所有方法都实现了显着控制。然而,在高接种量下,仅浸渍方法并不能显着控制大蒜白腐病。AgNPs 的应用改善了在田间条件下种植的洋葱和大蒜植物的鳞茎生长和产量。因此,AgNPs 可用作抗白腐病的纳米杀菌剂和洋葱和大蒜生产的纳米肥料。联合治疗比单独使用这些方法更有效。在田间试验中,无论是在菌核水平低还是高的田间,包括浸药和喷药在内的双重处理都显着降低了发病率。在低接种量下,使用所有方法都实现了显着控制。然而,在高接种量下,仅浸渍方法并不能显着控制大蒜白腐病。AgNPs 的应用改善了在田间条件下种植的洋葱和大蒜植物的鳞茎生长和产量。因此,AgNPs 可用作抗白腐病的纳米杀菌剂和洋葱和大蒜生产的纳米肥料。联合治疗比单独使用这些方法更有效。在田间试验中,无论是在菌核水平低还是高的田间,包括浸药和喷药在内的双重处理都显着降低了发病率。在低接种量下,使用所有方法都实现了显着控制。然而,在高接种量下,仅浸渍方法并不能显着控制大蒜白腐病。AgNPs 的应用改善了在田间条件下种植的洋葱和大蒜植物的鳞茎生长和产量。因此,AgNPs 可用作抗白腐病的纳米杀菌剂和洋葱和大蒜生产的纳米肥料。包括浸渍和喷洒处理,无论是在菌核水平低还是高的田间,都显着降低了疾病发生率。在低接种量下,使用所有方法都实现了显着控制。然而,在高接种量下,仅浸渍方法并不能显着控制大蒜白腐病。AgNPs 的应用改善了在田间条件下种植的洋葱和大蒜植物的鳞茎生长和产量。因此,AgNPs 可用作抗白腐病的纳米杀菌剂和洋葱和大蒜生产的纳米肥料。包括浸渍和喷洒处理,无论是在菌核水平低还是高的田间,都显着降低了疾病发生率。在低接种量下,使用所有方法都实现了显着控制。然而,在高接种量下,仅浸渍方法并不能显着控制大蒜白腐病。AgNPs 的应用改善了在田间条件下种植的洋葱和大蒜植物的鳞茎生长和产量。因此,AgNPs 可用作抗白腐病的纳米杀菌剂和洋葱和大蒜生产的纳米肥料。然而,在高接种量下,仅浸渍方法并不能显着控制大蒜白腐病。AgNPs 的应用改善了在田间条件下种植的洋葱和大蒜植物的鳞茎生长和产量。因此,AgNPs 可用作抗白腐病的纳米杀菌剂和洋葱和大蒜生产的纳米肥料。然而,在高接种量下,仅浸渍方法并不能显着控制大蒜白腐病。AgNPs 的应用改善了在田间条件下种植的洋葱和大蒜植物的鳞茎生长和产量。因此,AgNPs 可用作抗白腐病的纳米杀菌剂和洋葱和大蒜生产的纳米肥料。

更新日期:2021-07-13
down
wechat
bug