The electrochemical quantification of analytes in droplets of PBS separated by a fluorinated phase was investigated. PDMS-fused silica chips with pyrolysed photoresist electrodes were prepared using a simple fabrication technique and used to analyze droplets in flow. Potentiostatic chronoamperometry provided current readouts consistent with mass transport and the concentration inside the droplets. This paper highlights measurements of dopamine in droplets in T-junction microfluidic chips at unprecedently low concentrations, with a limit of detection of 207 nM and a linear range of 0.21–20 μM, giving results similar to continuous flow electrochemistry and allowing the analysis in the striatal extracellular range (<1 μM). The system was applied to the quick and reliable on-line detection of dopamine concentration steps in droplets collected with a microsampling probe in vitro, demonstrating the usefulness of the electrochemical device as a quantification module for microsampled chemicals in droplets.

中文翻译：

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使用热解光刻胶电极对液滴中的化学物质进行流式电化学检测：作为微量多巴胺定量模块的应用

研究了由氟化相分离的 PBS 液滴中分析物的电化学定量。使用简单的制造技术制备具有热解光刻胶电极的 PDMS 熔融石英芯片，并用于分析流动中的液滴。恒电位计时电流法提供与质量传输和液滴内浓度一致的电流读数。本文重点介绍了在前所未有的低浓度下测量 T 型接头微流控芯片液滴中多巴胺的方法，检测限为 207 nM，线性范围为 0.21–20 μM，结果类似于连续流电化学，并允许在纹状体细胞外范围 (<1 μM)。在体外，证明了电化学装置作为液滴中微采样化学物质的量化模块的有用性。