Basal cell carcinoma (BCC) and squamous cell carcinoma (SCC) are two predominant histological types of nonmelanoma skin cancer (NMSC), lacking effective early diagnostic markers. In this study, we assessed the diagnostic value of autoantibodies against p53, MMP-7, and Hsp70 in skin SCC and BCC. ELISA was performed to detect levels of autoantibodies in sera from 101 NMSC patients and 102 normal controls, who were recruited from the Cancer Hospital of Shantou University Medical College. A receiver operator characteristic curve was used to evaluate the diagnostic value. The serum levels of autoantibodies against p53, MMP-7, and Hsp70 were higher in NMSCs than those in the normal controls (all ). The AUC of the three-autoantibody panel was 0.841 (95% CI: 0.788-0.894) with the sensitivity and specificity of 60.40% and 91.20% when differentiating NMSCs from normal controls. Furthermore, measurement of this panel could differentiate early-stage skin cancer patients from normal controls (AUC: 0.851; 95% CI: 0.793-0.908). Data from Oncomine showed that the level of p53 mRNA was elevated in BCC (), and the Hsp70 mRNA was upregulated in SCC (). This serum three-autoantibody panel might function in assisting the early diagnosis of NMSC.

中文翻译：

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针对 p53、MMP-7 和 Hsp70 的自身抗体作为检测非黑色素瘤皮肤癌的潜在生物标志物

基底细胞癌 (BCC) 和鳞状细胞癌 (SCC) 是非黑色素瘤皮肤癌 (NMSC) 的两种主要组织学类型，缺乏有效的早期诊断标志物。在本研究中，我们评估了针对 p53、MMP-7 和 Hsp70 的自身抗体在皮肤 SCC 和 BCC 中的诊断价值。ELISA检测来自汕头大学医学院附属肿瘤医院的101名NMSC患者和102名正常对照血清中的自身抗体水平。接受者操作者特征曲线用于评估诊断价值。NMSCs 血清中针对 p53、MMP-7 和 Hsp70 的自身抗体水平高于正常对照组（所有）。三自身抗体组的 AUC 为 0.841（95% CI：0.788-0.894），区分 NMSCs 与正常对照时的敏感性和特异性分别为 60.40% 和 91.20%。此外，该组的测量可以区分早期皮肤癌患者和正常对照组（AUC：0.851；95% CI：0.793-0.908）。Oncomine 的数据显示，在 BCC 中 p53 mRNA 水平升高（），并且 Hsp70 mRNA 在 SCC 中上调（）。该血清三自身抗体组可能有助于 NMSC 的早期诊断。