ABSTRACT

Benzo[a]pyrene (BaP) is widely used as a reference compound when studying the toxicity of Polycyclic aromatic hydrocarbons (PAHs) to natural communities. In this study, 15d after exposure to BaP at the nominal concentrations of 5, 1 and 0.2 μg/L, liver, gill and mantle tissues of Crassostrea gigas and Mytilus coruscuswere taken for BaP uptake assessment, and Cytochrome P450 (CYP450) gene expression level and 7-ethoxyresorufifififin O-deethylase (EROD) activity testing, respectively. CYP450 gene expression level and EROD activity increased significantly with the increase of BaP exposure concentrations. The Significant correlations between BaP uptake and both CYP450 gene expression level and EROD activity indicates that they can be used as a suitable tools for the study of comprehensive levels of biomarker exposed to PAH. There were significant differences in the measurement results of these tissue antioxidant defenses parameters between different treatments.

摘要

在研究多环芳烃 (PAH) 对自然群落的毒性时，苯并 [a] 芘 (BaP) 被广泛用作参考化合物。在本研究中，在暴露于标称浓度为 5、1 和 0.2 μg/L 的 BaP 后 15 天，长牡蛎 和 贻贝的肝脏、鳃和外套膜组织分别用于 BaP 摄取评估和细胞色素 P450 (CYP450) 基因表达水平和 7-乙氧基试卤灵 O-脱乙基酶 (EROD) 活性测试。CYP450基因表达水平和EROD活性随着BaP暴露浓度的增加而显着增加。BaP 摄取与 CYP450 基因表达水平和 EROD 活性之间的显着相关性表明它们可以用作研究暴露于 PAH 的生物标志物综合水平的合适工具。这些组织抗氧化防御参数的测量结果在不同处理之间存在显着差异。