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Role of autophagy in midgut stem cells of silkworm Bombyx mori, during larval–pupal metamorphosis
Archives of Insect Biochemistry and Physiology ( IF 1.5 ) Pub Date : 2021-07-11 , DOI: 10.1002/arch.21832
Busra Gunay 1 , Ebru Goncu 1
Affiliation  

Autophagy is a critical mechanism for the self-renewal, proliferation, and differentiation of stem cells. Bombyx mori midgut has stem cells that play a role in the regeneration of the larval epithelium in larval stages and the formation of the pupal midgut epithelium during larval–pupal metamorphosis. In this study, the role of the autophagy mechanism in midgut stem cells during the formation of the pupal midgut was investigated. For this purpose, two different doses of autophagy inhibitor chloroquine were administered to B. mori larvae on days 7 and 8 of the fifth larval stage. Morphological changes during the formation process of the pupal epithelium, expression levels of autophagy-related genes Atg8 and Atg12 in stem cells, and the amounts of lysosomal enzyme acid phosphatase were determined after the application. The obtained findings were evaluated in comparison with the control groups. Abnormalities in the formation of the pupal midgut after inhibition of autophagy showed the significance of the autophagy mechanism during this period.

中文翻译:

自噬在家蚕中肠干细胞中的作用,在幼虫-蛹变态过程中

自噬是干细胞自我更新、增殖和分化的关键机制。家蚕中肠具有干细胞,其在幼虫阶段的幼虫上皮再生和幼虫-蛹变态过程中蛹中肠上皮的形成中发挥作用。在这项研究中,研究了自噬机制在蛹中肠形成过程中中肠干细胞中的作用。为此,在第五个幼虫阶段的第 7 天和第 8 天,向家蚕幼虫施用了两种不同剂量的自噬抑制剂氯喹。蛹上皮形成过程中的形态学变化、自噬相关基因Atg8Atg12 的表达水平在干细胞中,应用后测定溶酶体酶酸性磷酸酶的量。与对照组比较评估获得的结果。抑制自噬后蛹中肠形成的异常表明了这一时期自噬机制的重要性。
更新日期:2021-08-17
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