In this study, we cloned and identified melanoma differentiation-associated antigen 5 (MDA5), mitochondrial antiviral signalling protein (MAVS), TNF receptor-associated factor (TRAF3), TRAF family member-associated NF-kappa-B activator (TANK) and TANK-binding kinase 1 (TBK1) from turbot (Scophthalmus maximus). The smMAD5 encodes 996 amino acids containing two CARD domains at N-terminus, a DExD/H domain, a HELICc domain and a regulatory domain (RD) at the C-terminus. The smMAVS encodes 563 amino acids containing one CARD domain at N-terminus and one PVDQT motif, lacking proline-rich region in the middle and transmembrane domain (TM) at C-terminal. The smTRAF3 encodes 984 amino acids containing a ring finger domain and two zinc finger domains 1 and 2 and a MATH-TRAF3 domain. The smTANK encodes 341 amino acids containing a TBD superfamily domain. The smTBK1 encodes 721 amino acids containing a protein kinase domain, a ubiquitin-like domain (ULD) and a coiled–coil region. Immune responsive expression analysis showed that the five genes were all rapidly and significantly upregulated in vivo after poly (I:C) (LMW and HMW) and TRBIV challenge in spleen, gills, head kidney and muscle tissues. Our results indicate that the smMDA5, smMAVS, smTRAF3, smTANK and smTBK1 molecules play crucial role in antiviral immune response.

中文翻译：

---

大菱鲆RLR信号通路5个关键分子MDA5/MAVS/TRAF3/TANK/TBK1的分子克隆、鉴定及表达分析

在本研究中，我们克隆并鉴定了黑色素瘤分化相关抗原 5 (MDA5)、线粒体抗病毒信号蛋白 (MAVS)、TNF 受体相关因子 (TRAF3)、TRAF 家族成员相关 NF-κ-B 激活剂 (TANK) 和来自大菱鲆 ( Scophthalmus maximus ) 的TANK 结合激酶 1 (TBK1 )。所述smMAD5编码996个含有两个CARD结构域在N-末端，一个DExD / H结构域，HELICc结构域和在C端调节结构域（RD）的氨基酸。所述smMAVS编码563氨基酸含有N-末端一个CARD结构域和一个PVDQT基序，在C端的中间和跨膜结构域（TM）缺乏富含脯氨酸的区域的酸。该smTRAF3编码 984 个氨基酸，包含一个环指结构域和两个锌指结构域 1 和 2 以及一个 MATH-TRAF3 结构域。所述smTANK编码341个含有TBD超家族结构域的氨基酸。所述smTBK1编码含有蛋白激酶结构域，泛素样结构域（ULD）和coiled–线圈区域721个的氨基酸。免疫反应性表达分析表明，在脾、鳃、头肾和肌肉组织中聚 (I:C)（LMW 和 HMW）和 TRBIV 攻击后，这五个基因在体内均迅速且显着上调。我们的结果表明smMDA5、smMAVS、smTRAF3、smTANK和smTBK1 分子在抗病毒免疫反应中起关键作用。