Rosmarinic acid having potential anti-inflammatory and free radical scavenging activity. We examined the chemotherapeutic effect of rosmarinic against cisplatin (CIS)-induced ovarian toxicity via modulation of oxidative stress and inflammation. Swiss BALB mice used in experimental protocol and mice were divided into different groups. Intraperitoneal injection of CIS (7 mg/kg) was used for ovarian cancer induction. The rats were received rosmarinic acid (2.5, 5, and 10 mg/kg, body weight) treatment for 22 weeks. Body weight, ovary weight food, and water intake were estimated at regular time intervals. Hormonal and antioxidant parameters were estimated in the ovary tissue and serum at the end of the study. Cytokines, inflammatory, and apoptosis parameters were determined at the end of the study. Finally, the ovary tissue histopathology was performed at end of the experimental study. Rosmarinic acid significantly (p < 0.001) improved the body weight and reduced the ovary weight. Rosmarinic acid considerably reduced the hormonal assay parameters, such as antimullerian hormone, estradiol, luteinizing hormone, and follicle-stimulating hormone compared to model control mice. Rosmarinic treatment significantly (p < 0.001) reduced the level of nitric oxide, myeloperoxidase, and boosted the level of antioxidant parameters, such as glutathione, superoxide dismutase, catalase, and glutathione peroxidase in serum and ovary tissue. Rosmarinic acid downregulated the cytokines like interleukin-6, tumor necrosis factor-α, interleukin-1β; inflammatory parameter includes prostaglandin E₂, cyclooxygenase-2, and inducible nitric oxide synthase at a dose-dependently. Ovary tissue histopathology showed improvement after rosmarinic acid treatment. The result suggests that rosmarinic acid is a protective effect in ameliorating CIS-induced ovary toxicity via alteration of inflammatory and apoptosis parameters.

中文翻译：

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迷迭香酸通过抑制氧化应激和炎症减轻雌性小鼠顺铂引起的卵巢毒性


迷迭香酸具有潜在的抗炎和自由基清除活性。我们研究了迷迭香通过调节氧化应激和炎症对顺铂 (CIS) 诱导的卵巢毒性的化疗作用。实验方案中使用瑞士BALB小鼠，并将小鼠分为不同组。腹腔注射 CIS (7 mg/kg) 用于诱导卵巢癌。大鼠接受迷迭香酸（2.5、5和10 mg/kg体重）治疗22周。定期评估体重、卵巢重量、食物和水摄入量。在研究结束时评估了卵巢组织和血清中的激素和抗氧化参数。在研究结束时确定细胞因子、炎症和细胞凋亡参数。最后，在实验研究结束时进行卵巢组织组织病理学检查。迷迭香酸显着（ p < 0.001）改善体重并减轻卵巢重量。与模型对照小鼠相比，迷迭香酸显着降低了激素测定参数，例如抗苗勒氏管激素、雌二醇、黄体生成素和卵泡刺激素。迷迭香治疗显着（ p < 0.001）降低一氧化氮、髓过氧化物酶的水平，并提高血清和卵巢组织中抗氧化参数的水平，如谷胱甘肽、超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶。迷迭香酸下调白介素6、肿瘤坏死因子-α、白细胞介素1β等细胞因子；炎症参数包括前列腺素E ₂ 、环氧合酶-2和诱导型一氧化氮合酶，呈剂量依赖性。迷迭香酸治疗后卵巢组织病理学显示有所改善。 结果表明，迷迭香酸通过改变炎症和细胞凋亡参数，对改善 CIS 诱导的卵巢毒性具有保护作用。