Signal Transducer and Activator of Transcription 3 (STAT3) phosphorylation plays a key role in the transformation and proliferation of breast cancer cells. According to previous studies, STAT3 phosphorylation can be inhibited by curcumin in various cancer cells. Even so, its use is restricted due to low oral bioavailability. This study aimed to enhance curcumin solubility and bioavailability by loading it on Folic Acid-Selenium nanoparticles and determine their effect on STAT3 activation in breast cancer cell line. The synthesized nanoparticles were characterized by Fourier-transform infrared spectroscopy, Dynamic light scattering, and transmission electron microscope. MTT and western blotting methods were applied to determine the viability and protein level of MCF cell line. The size and zeta potential of nanoparticles were found to be about 130 nm and − 45 mV, respectively. The results indicated a reduction of 49.62 ± 1.85% (p < 0.05) in the viability and 79% (P < 0.0001) in the phospho-STAT3 (p-STAT3) level in the cell line tested. Curcumin-loaded folic acid conjugated nanoparticles could be an effective drug for breast cancer chemotherapy. Though, subsequent in vivo research is required to determine its efficiency and safety in clinical applications.

信号转导和转录激活因子 3 (STAT3) 磷酸化在乳腺癌细胞的转化和增殖中起关键作用。根据之前的研究，姜黄素可以抑制多种癌细胞中的STAT3磷酸化。即便如此，由于口服生物利用度低，其使用受到限制。本研究旨在通过将姜黄素加载到叶酸-硒纳米颗粒上来提高姜黄素的溶解度和生物利用度，并确定它们对乳腺癌细胞系中 STAT3 激活的影响。合成的纳米粒子通过傅里叶变换红外光谱、动态光散射和透射电子显微镜进行表征。应用MTT和蛋白质印迹方法来确定MCF细胞系的活力和蛋白质水平。发现纳米颗粒的尺寸和 zeta 电位分别约为 130 nm 和 - 45 mV。结果表明，在所测试的细胞系中，活力降低了 49.62 ± 1.85% (p < 0.05)，磷酸-STAT3 (p-STAT3) 水平降低了 79% (P < 0.0001)。负载姜黄素的叶酸偶联纳米颗粒可能是乳腺癌化疗的有效药物。但是，需要随后的体内研究来确定其在临床应用中的效率和安全性。