Background. This study is aimed at assessing the subsets of bone marrow macrophages in patients with myelodysplastic syndrome (MDS) and exploring the role of macrophages in the pathogenesis of MDS. Methods. Thirty-eight newly diagnosed MDS patients were enrolled in the Department of Hematology of General Hospital of Tianjin Medical University from June 2015 to June 2016. Bone marrow monocytes and macrophage subsets (M1/M2) were detected in patients with MDS and normal controls by flow cytometry. M1 macrophages were cultured in vitro, and the expression of IL-1β and TNF-α mRNA was measured using real-time polymerase chain reaction. Results. Compared with the normal control group, the proportion of bone marrow monocytes was higher ( vs. ), and the mean fluorescence intensity of surface molecule CD14 was lower in the higher-risk (HR) MDS group ( vs. , ). The ratio of M2 macrophages to monocytes was higher in patients with HR-MDS ( vs. , ). The ratio of M1 to M2 macrophages was lower in the HR-MDS group ( vs. , ). The expression of IL-1β and TNF-α mRNA in M1 macrophages was significantly lower in the MDS group (). Conclusions. Patients with MDS had abnormal macrophage polarization, which may be involved in the alteration of bone marrow microenvironments.

中文翻译：

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骨髓增生异常综合征患者的异常巨噬细胞极化

背景。本研究旨在评估骨髓增生异常综合征（MDS）患者的骨髓巨噬细胞亚群，并探讨巨噬细胞在MDS发病机制中的作用。方法。2015年6月至2016年6月天津医科大学总医院血液科新诊断MDS患者38例。通过流式检测MDS患者和正常对照的骨髓单核细胞和巨噬细胞亚群（M1/M2）细胞术。体外培养M1巨噬细胞，实时聚合酶链反应检测IL- 1β和TNF -αmRNA的表达。结果. 与正常对照组相比，骨髓单核细胞比例较高（对比），表面分子CD14的平均荧光强度在高风险（HR）MDS组中较低（对比, ）。HR-MDS 患者 M2 巨噬细胞与单核细胞的比例较高（对比, ）。HR-MDS 组 M1 与 M2 巨噬细胞的比例较低（对比, ）。MDS组M1巨噬细胞中IL- 1β和TNF -αmRNA的表达明显降低（）。 结论。MDS患者巨噬细胞极化异常，可能与骨髓微环境的改变有关。