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CsrA enters Hfq's territory: Regulation of a base-pairing small RNA
Molecular Microbiology ( IF 3.6 ) Pub Date : 2021-07-10 , DOI: 10.1111/mmi.14785
Thomas Søndergaard Stenum 1 , Erik Holmqvist 1
Affiliation  

Post-transcriptional regulatory networks in Gammaproteobacteria are to a large extent built around the two globally acting RNA-binding proteins (RBPs) CsrA and Hfq. Both RBPs interact with small regulatory RNAs (sRNAs), but the functional outcomes of these interactions are generally distinct. Whereas Hfq both stabilizes sRNAs and promotes their base-pairing to target mRNAs, the sRNAs bound by CsrA act as sequestering molecules that titrate the RBP away from its mRNA targets. In this issue of Molecular Microbiology, Lai et al. reveal that CsrA interacts with the Hfq-associated and base-pairing sRNA Spot 42. In this case, CsrA increases Spot 42 stability by masking a cleavage site for endoribonuclease RNase E, thereby promoting Spot 42-dependent regulation of srlA mRNA. Interestingly, the effect of CsrA on srlA expression is two-fold. In addition to affecting Spot 42-dependent regulation, CsrA directly inhibits translation of SrlM, an activator of srlA transcription. Together, this study reveals a new function for CsrA and indicates more intricate connections between the CsrA and Hfq networks than previously anticipated. Several recent studies have identified additional RBPs that interact with sRNAs. With new RBP identification methods at hand, it will be intriguing to see how many more sRNA-binding proteins will be uncovered.

中文翻译:

CsrA 进入 Hfq 的领域:碱基配对小 RNA 的调控

Gammaproteobacteria 的转录后调控网络在很大程度上是围绕两种全球作用的 RNA 结合蛋白 (RBP) CsrA 和 Hfq 构建的。两种 RBP 都与小调节 RNA (sRNA) 相互作用,但这些相互作用的功能结果通常是不同的。Hfq 既稳定 sRNA 又促进它们与靶 mRNA 的碱基配对,而与 CsrA 结合的 sRNA 充当隔离分子,将 RBP 从其 mRNA 靶标上滴定。在本期分子微生物学中,赖等人。揭示了 CsrA 与 Hfq 相关和碱基配对的 sRNA Spot 42 相互作用。在这种情况下,CsrA 通过掩盖核糖核酸内切酶 RNase E 的切割位点来增加 Spot 42 的稳定性,从而促进 srlA 的 Spot 42 依赖性调节mRNA。有趣的是,CsrA 对srlA表达的影响是双重的。除了影响 Spot 42 依赖性调节外,CsrA 还直接抑制 SrlM 的翻译,SrlM 是srlA转录的激活剂。总之,这项研究揭示了 CsrA 的一个新功能,并表明 CsrA 和 Hfq 网络之间的联系比以前预期的更复杂。最近的几项研究已经确定了与 sRNA 相互作用的其他 RBP。有了新的 RBP 识别方法,看看还有多少 sRNA 结合蛋白会被发现,这将是一件很有趣的事情。
更新日期:2021-07-10
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