The prevalence and levels of enteric viruses in untreated groundwater of private wells used for drinking and/or agricultural practices in rural Alberta were studied using the qPCR panel assay, integrated cell culture with qPCR and cell culture in the volume of 500 liters per sample through serial sampling. Seven viruses were assessed including adenovirus, rotavirus, norovirus, astrovirus, sapovirus, reovirus and JC virus. Five viruses were detected with an overall positive detection rate of 6.33 % (45 of 711 samples). The most frequently detected virus was adenovirus (48.9%, 22/45) followed by rotavirus (44.4%, 20/45), reovirus (20%, 9/45), JC virus (6.7%, 3/45) and norovirus (6.7%, 3/45). There was no significant difference in the positive detection rates, ranging from 1.1% to 3.4% by various well settings used for broiler farms, cow/calf farms, feedlots and rural acreages. Effects of well characteristics (aquifer type, well depth, static level of water, well seal) and well completion lithology on potential viral contamination of groundwater of private wells were also analyzed upon available data. The findings demonstrate that occurrence of enteric viruses is low and viral contamination is sporadic in groundwater of private wells in rural Alberta. Conventional fecal bacterial indicators (coliform and/or E. coli) were not a representative marker for viral contamination in groundwater wells in rural Alberta.

使用 qPCR 面板分析、qPCR 整合细胞培养和细胞培养，通过串行分析，对每个样本 500 升体积的细胞培养进行了研究，研究了艾伯塔农村地区用于饮用和/或农业实践的私人井未经处理地下水中肠道病毒的流行率和水平。采样。评估了七种病毒，包括腺病毒、轮状病毒、诺如病毒、星状病毒、萨波病毒、呼肠孤病毒和 JC 病毒。检测到五种病毒，总体阳性检出率为 6.33%（711 个样本中的 45 个）。最常检测到的病毒是腺病毒 (48.9%, 22/45)，其次是轮状病毒 (44.4%, 20/45)、呼肠孤病毒 (20%, 9/45)、JC 病毒 (6.7%, 3/45) 和诺如病毒 ( 6.7%，3/45）。阳性检出率没有显着差异，肉鸡养殖场、奶牛/小牛养殖场使用的各种井设置的阳性检出率从 1.1% 到 3.4% 不等，饲养场和农村土地。还根据现有数据分析了井特征（含水层类型、井深、静态水位、井封）和完井岩性对私人井地下水潜在病毒污染的影响。研究结果表明，肠道病毒的发生率很低，并且病毒污染在艾伯塔省农村私人井的地下水中是零星的。常规粪便细菌指标（大肠菌群和/或 研究结果表明，肠道病毒的发生率很低，并且病毒污染在艾伯塔省农村私人井的地下水中是零星的。常规粪便细菌指标（大肠菌群和/或 研究结果表明，肠道病毒的发生率很低，并且病毒污染在艾伯塔省农村私人井的地下水中是零星的。常规粪便细菌指标（大肠菌群和/或大肠杆菌) 不是阿尔伯塔省农村地下水井中病毒污染的代表性标志物。