ABSTRACT

Rice sprouts are reported to have potential as a functional food agent for health promotion, but studies on the biological activity of rice sprouts are insufficient. Thus, in this study, we investigated whether WERS exhibits immunostimulatory activity in macrophages. WERS increased the production of immunomodulatory factors such as nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumour necrosis factor-α (TNF-α) in RAW264.7 cells. In addition, WERS activated the phagocytosis of RAW264.7 cells. The inhibition of toll-like receptor 2 (TLR2) and toll-like receptor 4 (TLR4) blocked WERS-mediated production of immunomodulatory factors in RAW264.7 cells. In addition, the inhibition of p38 and JNK suppressed the production of immunomodulatory factors by WERS, and the activation of p38 and JNK was blocked by the inhibition of TLR2 and TLR4. Taken together, WERS induced macrophage activation through TLR2/4-dependent activation of p38 and JNK.

摘要

据报道，米芽具有作为促进健康的功能性食品剂的潜力，但对米芽生物活性的研究不足。因此，在这项研究中，我们调查了 WERS 是否在巨噬细胞中表现出免疫刺激活性。WERS 增加免疫调节因子的产生，例如一氧化氮 (NO)、诱导型一氧化氮合酶 (iNOS)、白介素-1β (IL-1β)、白介素-6 (IL-6) 和肿瘤坏死因子-α (TNF-α) ) 在 RAW264.7 单元格中。此外，WERS 激活了 RAW264.7 细胞的吞噬作用。Toll 样受体 2 (TLR2) 和 Toll 样受体 4 (TLR4) 的抑制阻止了 WERS 介导的 RAW264.7 细胞中免疫调节因子的产生。此外，p38 和 JNK 的抑制抑制了 WERS 免疫调节因子的产生，p38 和 JNK 的激活被 TLR2 和 TLR4 的抑制所阻断。总之，WERS 通过 p38 和 JNK 的 TLR2/4 依赖性激活诱导巨噬细胞激活。