The seeding of small juvenile green-lipped mussels (Perna canaliculus) onto coastal farms is associated with high losses. These losses can be reduced by seeding larger juveniles; however, the nursery culture of juveniles is unviable because of the high cost of producing live microalgal feeds. In this study, we compared the ability for two diets, a formulated microparticulate feed, MySpat and liposomes fabricated with mussel extract to replace live microalgae at different proportions for feeding small (1.9 mm shell length ±0.02 SE) green-lipped mussels. The experimental diets consisted of a mix of live Tisochrysis lutea, Diacronema lutheri and Chaetoceros muelleri that were replaced with increasing proportions of MySpat (25%, 50% and 75%), and liposomes (25% and 50%). There were no significant differences in mortality of mussels relative to the control (100% microalgae) among any of the diet treatments. However, mussel growth tended to decrease with increasing substitution of the microalgal component of the diets. The results from this study suggest that microparticulate and liposome formats provide a viable mode of food delivery to juvenile mussels, with improved formulation of these formats having the potential to lead to the substitution of live microalgal diets which make nursery culture unviable at present.

中文翻译：

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研究脂质体和微粒饲料在绿唇贻贝（Perna canaliculus）育苗中部分替代微藻的潜力

将小型绿唇贻贝 ( Perna canaliculus )幼苗接种到沿海养殖场会造成高损失。这些损失可以通过播种较大的幼鱼来减少；然而，由于生产活微藻饲料的成本高昂，幼鱼的苗圃养殖是不可行的。在本研究中，我们比较了两种饲料、配方微粒饲料、MySpat 和用贻贝提取物制成的脂质体以不同比例替代活微藻喂养小型（1.9 毫米壳长 ±0.02 SE）绿唇贻贝的能力。实验饲料包括活的黄体鞭毛虫、Diacronema lutheri和Chaetoceros muelleri的混合物。取而代之的是越来越多的 MySpat（25%、50% 和 75%）和脂质体（25% 和 50%）。在任何饮食处理中，贻贝的死亡率相对于对照（100% 微藻）没有显着差异。然而，随着日粮中微藻成分替代品的增加，贻贝的生长趋于下降。这项研究的结果表明，微粒和脂质体形式为幼年贻贝提供了一种可行的食物输送方式，这些形式的改进配方有可能导致替代活微藻饮食，这使得目前无法进行育苗养殖。