Abstract

Human lymphocytes as well as zebrafish embryos were used to study the toxicity of acrylamide, and the intervention effects of the dietary antioxidants (-)-epigallocatechin gallate and curcumin have been examined. The alkaline comet assay revealed that acrylamide (1, 5, 10 mmol/L) had dose-response effects on basic DNA damage and oxidative DNA damage in human lymphocytes. Exposure of zebrafish embryos to acrylamide resulted in decrease of hatching rate, and in increases of mortality and teratogenicity. (-)-Epigallocatechin gallate and curcumin reduced DNA damage and protected zebrafish embryos in the growth and development.

摘要

人类淋巴细胞和斑马鱼胚胎被用于研究丙烯酰胺的毒性，并检查了膳食抗氧化剂 (-)-表没食子儿茶素没食子酸酯和姜黄素的干预作用。碱性彗星试验表明，丙烯酰胺（1、5、10 mmol/L）对人淋巴细胞的基本 DNA 损伤和氧化性 DNA 损伤具有剂量反应效应。斑马鱼胚胎暴露于丙烯酰胺导致孵化率下降，死亡率和致畸性增加。(-)-Epigallocatechin gallate 和姜黄素减少 DNA 损伤并保护斑马鱼胚胎的生长和发育。