Polygoni Multiflori Radix Praeparata (PMRP) is used as Chinese herbal medicine with long history. However, reports about PMRP hepatotoxicity have increased recently, and producing area might be one reason. This article aims to figure out the relationship between producing area and hepatotoxic ingredients in PMRP. HPLC fingerprint for PMRP was established and the contents of gallic acid, trans-stilbene glycoside (TSG), emodin-8-O-β-D-glucoside (EG), emodin and physcion were determined. Clustering heatmap was implemented by TCMNPAS software，and principal component analysis was implemented by SPSS and SIMCA-P software. Hepatotoxic constituents’ contents of PMRP from separate producing area were different. PMRP from Guangxi had the highest content of gallic acid, TSG, EG, emodin and physcion, followed by Hubei, Guangdong, Guizhou, Yunnan. PMRP from Henan had the lowest contents of hepatotoxic components. Hepatotoxic components’ contents of PMRP in southern were higher than central China. This study carried out a preliminary qualitative and quantitative investigation on the PMRP from different producing places, which provided a basis for safe medication of PMRP.

中文翻译：

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不同产地何首乌中肝毒性成分的指纹图谱和HPLC测定

何首乌（PMRP）作为中药材，历史悠久。然而，关于 PMRP 肝毒性的报道最近有所增加，产地可能是原因之一。本文旨在弄清PMRP中产地与肝毒成分的关系。建立了PMRP的HPLC指纹图谱，测定了没食子酸、反式芪苷（TSG）、大黄素-8-O-β-D-葡萄糖苷（EG）、大黄素和大黄素的含量。聚类热图采用TCMNPAS软件实现，主成分分析采用SPSS和SIMCA-P软件实现。不同产地PMRP的肝毒性成分含量不同。广西PMRP中没食子酸、TSG、EG、大黄素和大黄素含量最高，其次是湖北、广东、贵州、云南。河南PMRP的肝毒性成分含量最低。南方地区PMRP的肝毒性成分含量高于华中地区。本研究对不同产地的PMRP进行了初步的定性和定量调查，为PMRP的安全用药提供了依据。