Depletion of coenzyme Q (CoQ) is associated with disease, ranging from myopathy to heart failure. To induce a CoQ deficit, C2C12 myotubes were incubated with high dose simvastatin. This resulted in a concentration-dependent inhibition of cell viability. Simvastatin-induced effects were prevented by co-incubation with mevalonic acid. When myotubes were incubated with 60 μM simvastatin, mitochondrial CoQ content decreased while co-incubation with CoQ nanodisks (ND) increased mitochondrial CoQ levels and improved cell viability. Incubation of myotubes with simvastatin also led to a reduction in oxygen consumption rate (OCR). When myotubes were co-incubated with simvastatin and CoQ ND, the decline in OCR was ameliorated. The data indicate that CoQ ND represent a water soluble vehicle capable of delivering CoQ to cultured myotubes. Thus, these biocompatible nanoparticles have the potential to bypass poor CoQ oral bioavailability as a treatment option for individuals with severe CoQ deficiency syndromes and/or aging-related CoQ depletion.

辅酶 Q (CoQ) 的消耗与疾病有关，从肌病到心力衰竭。为了诱导 CoQ 缺乏，C2C12 肌管与高剂量辛伐他汀一起孵育。这导致细胞活力的浓度依赖性抑制。通过与甲羟戊酸共孵育可防止辛伐他汀诱导的作用。当肌管与 60 μM 辛伐他汀一起孵育时，线粒体 CoQ 含量降低，而与 CoQ 纳米盘 (ND) 共孵育增加了线粒体 CoQ 水平并提高了细胞活力。用辛伐他汀孵育肌管也导致耗氧率 (OCR) 降低。当肌管与辛伐他汀和 CoQ ND 共同孵育时，OCR 的下降得到改善。数据表明 CoQ ND 代表一种水溶性载体，能够将 CoQ 输送到培养的肌管。因此，