A feeding experiment was conducted to survey proper microalgae species for non-fish meal and non-fish oil diet of red sea bream. Five iso-nitrogenous and iso-lipidic diets were formulated (CP 47%, CL 19%). Fish oil formulated non-fish meal diet was arranged as a control diet with soybean meal, soy protein concentrate and corn gluten meal (FO). Fish oil of FO was replaced by Nannochloropsis meal and Schizochytrium meal for each of two diets (NAN, SCH). Both microalgae meals were mixed in a diet (NS), and Chlorella meal was formulated in NS diet to replace plant protein source (NSC). Duplicated groups of juvenile red sea bream (5.9 g) were fed the experimental diets for 75 days to near satiation. The growth was the highest in NS and NSC diet group. The other algae meal formulated diets fed groups showed the similar growth performance to FO. In fatty acid profiles, NAN group showed the lowest n-3 LC-PUFA with low DHA content in fatty acid composition of total lipid in fish carcass and hepatic PL, but the other algae meal diet group showed no significant difference with FO group. The results might suggest blending microalgae and using them with high amount is a key strategy for development of non-fish meal and non-fish oil diet for red sea bream.

中文翻译：

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微藻作为鱼饲料的主要成分：通过混合微藻 Nannochloropsis、Chlorella 和 Schizochytrium 为红鲷鱼开发非鱼粉和非鱼油饲料

进行了一项饲养实验，以调查适合红鲷鱼非鱼粉和非鱼油饮食的微藻种类。配制了五种等氮和等脂饮食（CP 47%，CL 19%）。鱼油配制的非鱼粉日粮被安排为含有豆粕、大豆浓缩蛋白和玉米蛋白粉 (FO) 的对照日粮。对于两种饮食 (NAN, SCH) 中的每一种，FO 的鱼油都被Nannochloropsis粉和Schizochytrium粉代替。两种微藻粉混合在饮食 (NS) 中，小球藻在 NS 饮食中配制膳食以替代植物蛋白源 (NSC)。重复组的幼鱼 (5.9 g) 被喂食实验饮食 75 天至接近饱食。NS和NSC饮食组的增长最高。其他藻类粉配方饲料喂养组显示出与 FO 相似的生长性能。在脂肪酸谱中，NAN 组显示出最低的 n-3 LC-PUFA 和低 DHA 含量在鱼尸体和肝脏 PL 的总脂质脂肪酸组成中，但其他藻粉饮食组与 FO 组没有显着差异。结果可能表明混合微藻并大量使用它们是开发红鲷非鱼粉和非鱼油饮食的关键策略。