This study aimed to evaluate the influence of microalgae addition on the physical and functionality of bioflocs and the growth and health performance of giant prawn (Macrobrachium rosenbergii). Three biofloc-based giant prawn culture systems were compared that is biofloc system without microalgae addition as a control, biofloc system with Chlorella sp. addition and biofloc system with Ankistrodesmus sp. addition. Giant prawn juveniles (2.00 ± 0.10 g) were randomly distributed into 12 units of outdoor fibre tanks (500 L in capacity) at a density of 40 juvenile per tank and maintained for 60 days. Molasses was added at an estimated C/N ratio of 6, and each microalga was added once a week at a density of about 10⁵ cell/mL. The addition of microalgae in biofloc systems did not affect the prawn's survival and immune parameters as well as its robustness against hypoxia stress (p > 0.05). Alkalinity in the microalgae-added biofloc systems was significantly higher (p < 0.05) than that of the control, whereas total ammonia nitrogen (TAN), nitrite, nitrate, total phosphate and dissolved phosphate were not significantly different amongst the treatments. The addition of Chlorella sp. and Ankistrodesmus sp. resulted in 11% and 18% higher size of biofloc particles, respectively, and altered the fatty acid profile of bioflocs, but did not affect the protein content. In conclusion, Chlorella sp. and Ankistrodesmus sp. addition could increase the particle size and alter the fatty acid profile of bioflocs, but did not affect the water quality and the growth performance of giant prawn in biofloc systems.

中文翻译：

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小球藻的评估。和 Ankistrodesmus sp。对大虾养殖中生物絮团系统性能的补充

本研究旨在评估添加微藻对生物絮团的物理和功能以及罗氏沼虾（Macrobrachium rosenbergii）生长和健康性能的影响。比较了三种基于生物絮团的巨型对虾养殖系统，即不添加微藻的生物絮团系统作为对照，带有小球藻的生物絮团系统。除了和生物絮凝系统Ankistrodesmus SP。添加。将巨虾幼虾（2.00 ± 0.10 g）以每缸40 条幼虾的密度随机分配到12 个室外纤维池（容量500 L）中，并维持60 天。糖蜜以估计的 C/N 比为 6 添加，每种微藻每周添加一次，密度约为 10 ⁵细胞/毫升。在生物絮团系统中添加微藻不影响对虾的存活和免疫参数以及对缺氧胁迫的鲁棒性（p > 0.05）。添加微藻的生物絮团系统的碱度显着高于对照 ( p < 0.05)，而总氨氮 (TAN)、亚硝酸盐、硝酸盐、总磷酸盐和溶解磷酸盐在处理之间没有显着差异。添加小球藻。和Ankistrodesmus sp。导致生物絮团颗粒尺寸分别增加 11% 和 18%，并改变了生物絮团的脂肪酸谱，但不影响蛋白质含量。总之，小球藻属。和Ankistrodesmussp. 添加可以增加颗粒大小并改变生物絮团的脂肪酸谱，但不会影响生物絮团系统中大虾的水质和生长性能。