Our aim was to assess the blood physiology and antioxidant and immune responses of the olive flounder (Paralichthys olivaceus) following pH changes in the water. P. olivaceus (mean weight 95.7 ± 6.5 g and mean length 21.4 ± 1.3 cm) were exposed to the different pH levels (pH 5, 6, 7, 8, 9, and 10) for 2 weeks. Blood physiology parameters, such as the levels of hematocrit, magnesium, glucose, glutamic oxalate transaminase (GOT), glutamic pyruvate transaminase (GPT), and alkaline phosphatase (ALP) were significantly altered by low (acidic) pH or high (alkaline) pHs. With regard to antioxidant responses, the superoxide dismutase (SOD) and glutathione S-transferase (GST) activities in the liver were significantly altered by the pH changes, but there were no significant changes of these activities in the gill tissues. The immunoglobulin M levels increased significantly at pH 10 after 2 weeks. The results of this study suggest that pH changes (acidity: < pH 6, alkalinity:> pH 9) affect blood physiology, as well as antioxidant and immune responses in P. olivaceus.

我们的目的是评估橄榄比目鱼 ( Paralichthys olivaceus ) 在水中 pH 值变化后的血液生理学以及抗氧化和免疫反应。P. olivaceus（平均重量 95.7 ± 6.5 克和平均长度 21.4 ± 1.3 厘米）暴露于不同的 pH 值水平（pH 值 5、6、7、8、9 和 10）2 周。血液生理参数，例如血细胞比容、镁、葡萄糖、谷氨酸草酸转氨酶 (GOT)、谷氨酸丙酮酸转氨酶 (GPT) 和碱性磷酸酶 (ALP) 的水平因低（酸性）pH 或高（碱性）pH 值而显着改变. 在抗氧化反应方面，肝脏中的超氧化物歧化酶 (SOD) 和谷胱甘肽 S-转移酶 (GST) 活性因 pH 值变化而显着改变，但鳃组织中的这些活性没有显着变化。2 周后，免疫球蛋白 M 水平在 pH 10 时显着增加。这项研究的结果表明 pH 值变化（酸度：< pH 6，碱度：> pH 9）会影响血液生理，P. olivaceus。