Abstract

Poly(catechin) (p(CAT)) nanoparticles (NPs), 173 ± 4 nm was prepared as a therapeutic agent with hydrolytic degradability affording sustainable CAT release over 20 d at carcinogenic conditions, pH 5.5 and 37.5 °C. Cell viability studies on MC38 colon cancer cells revealed the anticancer potential of p(CAT) NPs with 691 µg/mL IC₅₀ value while being well-tolerated by nonmalignant CCD841 CoN colon cells at 72 h incubation-time. P(CAT) NPs showed effective antioxidant capacity with 241 ± 7 and 456 ± 54 µg/mL of GA equivalency of total phenol content (TPC), and total flavonoid content (TFC) values and 1.19 ± 0.8 µmol/g Trolox equivalent antioxidant capacity by 2,2'-Azino-bis-(3-ethylbenzothioazoline-6-sulfonic acid (ABTS^•+) scavenging assay.

摘要

制备了 173 ± 4 nm 的聚（儿茶素）（p（CAT））纳米颗粒（NPs）作为具有水解降解能力的治疗剂，在致癌条件下（pH 5.5 和 37.5 °C）可在 20 天内提供可持续的 CAT 释放。对 MC38 结肠癌细胞的细胞活力研究表明，p(CAT) NPs 具有 691 µg/mL IC ₅₀值的抗癌潜力，同时在 72 小时的孵育时间内被非恶性 CCD841 CoN 结肠细胞良好耐受。P(CAT) NPs 显示出有效的抗氧化能力，总酚含量 (TPC) 和总黄酮含量 (TFC) 值的 GA 等效值为 241 ± 7 和 456 ± 54 µg/mL，抗氧化能力等效于 1.19 ± 0.8 µmol/g通过 2,2'-Azino-bis-(3-ethylbenzothioazoline-6-sulfonic acid (ABTS ^•+ ) 清除试验。