The worldwide loss of date palm (Phoenix dactylifera L.) productivity is due to the infestation of the red pest weevil (RPW). The pest makes tunnels in the tree trunk and could be followed by secondary microbial infections causing oozing of fluid with a distinct fermentation odor. This study aimed at isolation and identification of secondary invader bacteria associated with the RPW and confirmation of their potential destructive effect on the tree. Four bacterial isolates were recovered from the infested date palm tissues, and they were identified based on phenotypic characteristics using the VITEK2 system. Three out of the four isolates were identified as Pseudomonas aeruginosa, and one isolate was identified as Burkholderia cepacia. All isolates caused rot of date palm germinated seeds (up to 40%), and wilt and root rot of date palm seedlings (40–100%). Pseudomonas aeruginosa (isolate No. 3) was the most aggressive isolate that involved 40% mortality and caused 100% of root rot incidence in date palm seedlings. Burkholderia cepacia showed the lowest degree of rot on the germinated seeds, percentage mortality, percentage root rot incidence of date palm seedlings. Pseudomonas aeruginosa (isolate No. 1) and B. cepacia caused a high loss in the weight of date palm petioles tissues (about 10%). The study threw light on the detrimental effects of secondary invader microbes associated with the RPW infestation. The study recommended an application of an integrated management program containing both antibacterial and insecticidal preparation in the management of the RPW to avoid a further negative effect on the palm trees.

中文翻译：

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与椰枣树红色害虫象鼻虫侵染相关的二次入侵细菌

世界范围内枣椰树 ( Phoenix dactylifera L.) 生产力的丧失是由于红色害虫象鼻虫 (RPW) 的侵染。害虫在树干上挖洞，随后可能会发生继发性微生物感染，导致液体渗出，散发出明显的发酵气味。本研究旨在分离和鉴定与 RPW 相关的二次入侵细菌，并确认它们对树木的潜在破坏作用。从受侵染的椰枣组织中回收了四种细菌分离株，并使用 VITEK2 系统根据表型特征对它们进行了鉴定。4株分离物中的3株被鉴定为铜绿假单胞菌，1株被鉴定为洋葱伯克霍尔德菌。. 所有分离株都会导致枣椰发芽种子腐烂（高达 40%），以及枣椰幼苗的枯萎和根腐病（40-100%）。铜绿假单胞菌（第 3 号分离株）是最具侵袭性的分离株，死亡率为 40%，导致椰枣幼苗的根腐病发生率为 100%。洋葱伯克霍尔德菌对发芽种子的腐烂程度、死亡率、枣椰幼苗的根腐病发生率最低。铜绿假单胞菌（第 1 号分离株）和洋葱芽孢杆菌导致椰枣叶柄组织重量大幅下降（约 10%）。该研究揭示了与 RPW 感染相关的二次入侵微生物的有害影响。该研究建议在 RPW 管理中应用包含抗菌和杀虫制剂的综合管理计划，以避免对棕榈树产生进一步的负面影响。