Abstract

Recently, several scientific groups investigated the suitable enzymatic route for synthesis of structured phenolic lipids for cosmetic and nutritive purposes. The feruloylated palm stearin (FPS) was obtained from enzymatic transesterification reactions of palm stearin with ferulic acid in solvent-free media with 1 mmol of glycerol or without it, using non-specific lipases, NovoCor ADL (Candida antarctica lipase A); Novozyme435^® (Candida antarctica lipase B), and 1,3-specific lipase Lipozyme TL 100L from Thermomyces lanuginosus. The individual lipases and their combinational systems were examined. ESI⁺-MS/MS confirmed the formation of 19 feruloylated compounds. FPSs were produced by all lipases and their combinations. The positive synergetic effect occurred for 1,3-specific lipase and unspecified lipases with mass ratio 8:2, respectively. The antioxidant activities of all FPSs were examined. FPS produced by NovoCor ADL lipase in presence of free glycerol showed an antioxidant activity 10.3-times higher. In conclusion, the two low-cost lipases NovoCor ADL and Lipozyme TL 100L and its combi-lipase system could be considered suitable low-cost alternative to the expensive wide use N435 lipase in the solvent free production process of FPS for food and cosmetic applications.

摘要

最近，几个科学小组研究了用于合成用于化妆品和营养目的的结构化酚类脂质的合适酶促途径。阿魏酸化棕榈硬脂 (FPS) 是使用非特异性脂肪酶 NovoCor ADL ( Candida antarctica脂肪酶 A)，在含有或不含 1 mmol 甘油的无溶剂介质中，棕榈硬脂与阿魏酸的酶促酯交换反应获得的；Novozyme435 ^®（南极念珠菌脂肪酶 B）和来自Thermomyces lanuginosus的 1,3 特异性脂肪酶 Lipozyme TL 100L 。检查了单个脂肪酶及其组合系统。ESI ⁺-MS/MS 证实了 19 种阿魏酰化化合物的形成。FPS 由所有脂肪酶及其组合产生。1,3-特异性脂肪酶和质量比为8:2的非特异性脂肪酶分别产生了积极的协同作用。检查了所有 FPS 的抗氧化活性。NovoCor ADL 脂肪酶在游离甘油存在下生产的 FPS 显示出高出 10.3 倍的抗氧化活性。总之，两种低成本的脂肪酶 NovoCor ADL 和 Lipozyme TL 100L 及其组合脂肪酶系统可以被认为是用于食品和化妆品应用的 FPS 无溶剂生产过程中昂贵且广泛使用的 N435 脂肪酶的合适的低成本替代品。