The melting of glaciers due to global warming leads to the formation of proglacial water bodies such as streams and lakes in their downstream environment. Triloknath glacier in the North-Western Himalayas has retreated unprecedently at a rate of 180 m /decade, forming similar water bodies. Glacier meltwater is considered the primary source of microbial diversity in downstream water bodies. Documentation of such diversity is crucial to retrieve the specialized microbial population because of the shift in the aquatic system due to deglaciation. In the current study, bacterial community structure based on V3-V4 region amplicons of 16S rRNA gene of two proglacial lakes and the glacial stream of Triloknath glacier was compared. Proglacial lake I site represents the ice-contacted proglacial lake while the proglacial lake II represents an ice-distal proglacial lake generally said to be evolved from the former. Proteobacteria was the most abundant phylum across the three sites. The prevalence of Proteobacteria in the proglacial lake I, proglacial lake II and glacial stream was 43.58%, 49.91%, and 40.08%, respectively. The highest species richness based on Chao I index (656) was observed in glacial stream while, the highest species diversity based on the Shannon index (8.95) was observed in proglacial lake I. The physiochemical conditions of glacial stream and proglacial lake II were closer than the lake I. These findings were corroborated with microbial diversity results as beta diversity analysis based on the Bray-curtis matrix indicated the closeness of glacier stream and proglacial lake II ecosystem and these sites also shared maximum bacterial genera.16S rRNA gene-based functional analysis showed the occurrence of xenobiotics degrading genes exposing the presence of pollutants in glacier ecosystem. The current study generated baseline information about the change in bacterial diversity due to deglaciation and hydrological connectivity of downstream water bodies with the glacier. The study documented residential microbial population of sensitive ecological niche of proglacial water bodies of Triloknath glacier ecosystem.

由于全球变暖导致冰川融化，导致下游环境中形成河流和湖泊等冰川前水体。喜马拉雅西北部的 Triloknath 冰川以 180 m/decade 的速度前所未有地退缩，形成了类似的水体。冰川融水被认为是下游水体微生物多样性的主要来源。由于冰川消退导致水生系统发生变化，因此记录这种多样性对于恢复专门的微生物种群至关重要。本研究比较了基于两个前冰湖和Triloknath冰川冰河16S rRNA基因V3-V4区扩增子的细菌群落结构。原冰湖Ⅰ站点代表与冰接触的原冰湖，而原冰湖Ⅱ代表一般认为是由前者演化而来的远冰前冰湖。Proteobacteria 是三个站点中最丰富的门。Proteobacteria在前冰湖I、前冰湖II和冰河中的流行率分别为43.58%、49.91%和40.08%。以Chao I指数为基础的物种丰富度最高（656）在冰川河流中，而以香农指数为基础的物种多样性最高（8.95）在冰河湖I中观察到。冰河与冰河湖II的理化条件更接近比湖I。这些发现得到了微生物多样性结果的证实，因为基于 Bray-curtis 矩阵的 Beta 多样性分析表明冰川流和前冰湖 II 生态系统的接近性，并且这些地点也共享最大的细菌属。基于 16S rRNA 基因的功能分析表明外源性降解基因暴露了冰川生态系统中污染物的存在。目前的研究产生了关于由于冰川消融和下游水体与冰川的水文连通性引起的细菌多样性变化的基线信息。该研究记录了 Triloknath 冰川生态系统前冰水体敏感生态位的居住微生物种群。基于 16S rRNA 基因的功能分析表明，外源降解基因的出现暴露了冰川生态系统中污染物的存在。目前的研究产生了关于由于冰川消融和下游水体与冰川的水文连通性引起的细菌多样性变化的基线信息。该研究记录了 Triloknath 冰川生态系统前冰水体敏感生态位的居住微生物种群。基于 16S rRNA 基因的功能分析表明，外源降解基因的出现暴露了冰川生态系统中污染物的存在。目前的研究产生了关于由于冰川消融和下游水体与冰川的水文连通性引起的细菌多样性变化的基线信息。该研究记录了 Triloknath 冰川生态系统前冰水体敏感生态位的居住微生物种群。