Biobeds are agriculture-based bioremediation tools used to safely contain and microbially degrade on-farm pesticide waste and rinsate, thereby reducing the negative environmental impacts associated with pesticide use. While these engineered ecosystems demonstrate efficient pesticide removal, the microbiomes in these environments remain largely understudied both taxonomically and functionally. This study used metagenomic and metatranscriptomic techniques to characterize the microbial community in a two-cell Canadian biobed system before and after a field season of pesticide application. These culture-independent approaches identified an enrichment of xenobiotic-degrading bacteria, such as Afipia, Sphingopyxis and Pseudomonas, and enrichment and transcription of xenobiotic-degrading genes, such as peroxidases, oxygenases, and hydroxylases, among others; we were able to directly link the transcription of these genes to Pseudomonas, Oligotropha, Mesorhizobium, Rhodopseudomonas, and Stenotrophomonas taxa.

中文翻译：

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宏基因组和宏转录组学分析揭示了加拿大草原双细胞生物床系统中异种生物降解细菌专家和异种生物降解基因的富集

生物床是基于农业的生物修复工具，用于安全地容纳和微生物降解农场农药废物和冲洗液，从而减少与农药使用相关的负面环境影响。虽然这些工程生态系统显示出有效的杀虫剂去除，但这些环境中的微生物组在分类学和功能上仍未得到充分研究。本研究使用宏基因组学和宏转录组学技术来表征加拿大两细胞生物床系统在农药施用季节前后的微生物群落特征。这些与培养无关的方法确定了异种生物降解细菌的富集，例如Afipia、Sphingopyxis和Pseudomonas以及外源性降解基因的富集和转录，例如过氧化物酶、加氧酶和羟化酶等；我们能够直接将这些基因的转录与假单胞菌属、寡养菌属、中根瘤菌属、红假单胞菌属和窄食单胞菌属类群联系起来。