Introduction. Outbreaks of carbapenem-resistant A. baumannii and A. nosocomialis have occurred worldwide in healthcare settings. Rapid and reliable molecular typing of bacterial isolates is vital for the effective surveillance of institutional outbreaks. The Pan-PCR and OXA-PCR assays are two multiplex PCR-based assays for the molecular typing of Acinetobacter species. Gap statement. However, few studies have investigated the discriminatory power of two multiplex PCR assays in in the genotyping of Acinetobacter species. Aim. We aimed to evaluate the efficacies of the Pan-PCR and OXA-PCR assays for molecular typing of A. baumannii and A. nosocomialis . Methodology. A total of 105 carbapenem-resistant A. baumannii isolates (CRABs) and 93 carbapenem-resistant A. nosocomialis isolates (CRANs) obtained from blood cultures were used for molecular typing by the Pan-PCR and OXA-PCR assays and two multilocus sequence typing (MLST) schemes. Results. The isolates were individually divided into 12 and 21 different sequence types via the Pasteur and Oxford MLST schemes, respectively. Additionally, these isolates were distinguished into 18 different types by the Pan-PCR and OXA-PCR assays. The results of the Pan-PCR and OXA-PCR assays distinguished CRABs and CRANs with a sensitivity of 98.13 % and a specificity of 100 %. Conclusion. The Pan-PCR and OXA-PCR assays are promising alternative methods for rapid molecular typing of CRABs and CRANs in a routine laboratory setting.

中文翻译：

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通过多重 Pan-PCR 和 OXA-PCR 检测快速分型耐碳青霉烯鲍曼不动杆菌和院内不动杆菌

介绍。耐碳青霉烯类鲍曼不动杆菌和院内曲霉的爆发已在全球范围内的医疗机构中爆发。细菌分离株的快速可靠的分子分型对于有效监测机构暴发至关重要。Pan-PCR 和 OXA-PCR 检测是两种基于多重 PCR 的检测，用于不动杆菌属物种的分子分型。间隙声明。然而，很少有研究调查两种多重 PCR 检测在不动杆菌属物种基因分型中的区分能力。目标。我们旨在评估 Pan-PCR 和 OXA-PCR 检测对鲍曼不动杆菌和 A. 医院内感染 。方法。总共有105耐碳青霉烯类鲍曼不动杆菌菌株（螃蟹）和93耐碳青霉烯类A. nosocomialis从血培养物中获得分离株（克莱恩）通过泛PCR和OXA-PCR测定和两个多位点序列分用于分子分(MLST) 计划。结果。分离株分别通过巴斯德和牛津 MLST 方案分别分为 12 和 21 种不同的序列类型。此外，通过 Pan-PCR 和 OXA-PCR 分析将这些分离株分为 18 种不同类型。Pan-PCR 和 OXA-PCR 分析的结果区分了 CRAB 和 CRAN，灵敏度为 98.13%，特异性为 100%。结论。 Pan-PCR 和 OXA-PCR 检测是在常规实验室环境中快速对 CRAB 和 CRAN 进行分子分型的有前途的替代方法。