The protein deacetylase sirtuin 1 (SIRT1) and adenosine monophosphate-dependent protein kinase (AMPK) play important roles in the development of insulin resistance. In glomerular podocytes, crosstalk between these two enzymes may be altered under hyperglycemic conditions. SIRT1 protein levels and activity and AMPK phosphorylation decrease under hyperglycemic conditions, with concomitant inhibition of the effect of insulin on glucose uptake into these cells. Nitric oxide (NO)-dependent regulatory signaling pathways have been shown to be downregulated under diabetic conditions. The present study examined the involvement of the NO synthase (NOS)/NO pathway in the regulation of SIRT1-AMPK signaling and glucose uptake in podocytes.

中文翻译：

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一氧化氮合酶/一氧化氮途径参与足细胞 SIRT1-AMPK 串扰的调节：对葡萄糖摄取的影响


蛋白质脱乙酰酶 Sirtuin 1 (SIRT1) 和腺苷单磷酸依赖性蛋白激酶 (AMPK) 在胰岛素抵抗的发展中发挥重要作用。在肾小球足细胞中，这两种酶之间的串扰可能在高血糖条件下发生改变。在高血糖条件下，SIRT1 蛋白水平和活性以及 AMPK 磷酸化降低，同时抑制胰岛素对这些细胞摄取葡萄糖的影响。一氧化氮 (NO) 依赖性调节信号通路已被证明在糖尿病条件下下调。本研究探讨了 NO 合酶 (NOS)/NO 通路在足细胞中 SIRT1-AMPK 信号传导和葡萄糖摄取调节中的参与情况。