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Effect of L-Hyp supplementation on collagen muscle histology, gene expression, growth performance, body composition and fillet texture on big size European sea bass (Dicentrarchux labrax)
Aquaculture Reports ( IF 3.2 ) Pub Date : 2021-07-09 , DOI: 10.1016/j.aqrep.2021.100787
Pedro L. Castro 1 , Sergio Plasencia 1 , María Jesús Zamorano 1 , Luis Guerrero 2 , Anna Claret 2 , José A. Beltrán 3 , Juan Calanche 3 , Rafael Ginés 1
Affiliation  

Hydroxyproline (L-Hyp) is amply present in fishmeal but limited in plant-protein sources. This study was conducted to evaluate the effects of supplementation with dietary L-Hyp on the distribution of collagen types in the muscle, and on the texture, survival rate, growth rate, feed utilization, body composition as well as the expression of the gene that encodes the pro-alpha2 chains of type I collagen (Col Iα2) of large European sea bass (initial body weight 609.21 ± 75.39 g) from high plant-protein diets. Four isoproteic (42 % crude protein) and isolipidic (20 % crude lipid) experimental diets were formulated adding 0.6 (HL diet), 1.2 (HM diet), and 2% (HH diet) L-Hyp, respectively. Three periods of feeding of 45, 99 and 143 days were studied. L-Hyp supplementation at 1.2 % and 2 % significantly improved specific growth rate (SGR) and feed conversion ratio (FCR). In the white muscle, type I, IV collagen and trichromic stain were significantly higher in HH feed than the control diet. In the red muscle, only type I collagen was higher. HH diet, also increases Col Iα2 mRNA levels in muscle significantly. It can be concluded that the addition of crystalline l-Hyp at 1.2 % or 2 % in high plant-protein diets indicates positive effects on growth performance of adult European sea bass and increase in muscle total collagen deposition.



中文翻译:

补充 L-Hyp 对大尺寸欧洲鲈鱼 ( Dicentrarchux labrax ) 的胶原蛋白肌肉组织学、基因表达、生长性能、身体成分和鱼片质地的影响

羟脯氨酸 (L-Hyp) 大量存在于鱼粉中,但在植物蛋白来源中受到限制。本研究旨在评估补充膳食 L-Hyp 对肌肉中胶原蛋白类型分布的影响,以及对肌肉质地、成活率、生长率、饲料利用率、身体成分以及基因表达的影响。编码来自高植物蛋白饮食的大型欧洲鲈鱼(初始体重 609.21 ± 75.39 g)的 I 型胶原蛋白(Col Iα2)的 pro-alpha2 链。分别添加 0.6(HL 饮食)、1.2(HM 饮食)和 2%(HH 饮食)L-Hyp 配制四种等蛋白(42% 粗蛋白)和异脂(20% 粗脂质)实验饮食。研究了 45、99 和 143 天的三个喂养周期。L-Hyp 补充剂 1。2 % 和 2 % 显着提高了比生长率 (SGR) 和饲料转化率 (FCR)。在白肌中,HH 饲料中的 I、IV 型胶原蛋白和三色染色显着高于对照饮食。在红肌中,只有 I 型胶原蛋白较高。HH 饮食也会显着增加肌肉中的 Col Iα2 mRNA 水平。可以得出结论,加入结晶在高植物蛋白饮食中添加 1.2% 或 2% 的l -Hyp 表明对成年欧洲鲈鱼的生长性能和肌肉总胶原沉积增加有积极影响。

更新日期:2021-07-09
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