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Production and analysis of capsules containing microorganisms consortiated for future application in petroleum bioremediation
Biodegradation ( IF 3.1 ) Pub Date : 2021-07-09 , DOI: 10.1007/s10532-021-09956-9
Douglas Faria 1 , Grazielle Dias Machado 1 , Rodrigo de Abreu Lang 1 , Fernando Santos 2 , Rogério Lourega 3
Affiliation  

Oil spills cause severe environmental and economic impacts, so the use of bioremediation techniques has been widely studied to solve this problem. Due to its complex composition, the oil prevents the full action of microorganisms, and in this way, the microbial consortium encapsulation technique is an innovation in the use of bacteria and biomass in the face of possible oil degradation, with the possibility of overcoming techniques such as bio-enhancement and biostimulation in the face of factors such as nutrient availability, oxygenation and temperature. Therefore, this work aims to produce capsules containing microbiological consortium and analyze its characteristics using the techniques TGA, DSC, FESEM, viable cell count, emulsification index and surface tension, in order to propose the best conditions to be applied. TGA and DSC results showed that the capsules have thermal stability in the range of 25–40 °C. Viable cell counts were more effective in capsules containing 1% (w/v) sodium alginate, and the emulsification index showed a large increase (80%) from day 5, as well as surface tension had a large drop (48%) in the same period. The increase in the emulsification index is caused by the increase in the production of biosurfactants (amphipathic molecules) by the bacteria consortium and this offers a greater contact between the microorganisms and the oil, providing best conditions for the degradation of oil. Therefore, all analyzes showed excellent results for future application in oil spills.



中文翻译:

含有微生物的胶囊的生产和分析,未来在石油生物修复中的应用

石油泄漏会造成严重的环境和经济影响,因此已广泛研究使用生物修复技术来解决这个问题。由于其复杂的成分,油阻止了微生物的充分作用,因此,微生物联合体封装技术是在面对可能的油降解时利用细菌和生物质的创新,有可能克服诸如此类的技术。面对营养供应、氧合和温度等因素时的生物增强和生物刺激。因此,本工作旨在通过TGA、DSC、FESEM、活细胞计数、乳化指数和表面张力等技术生产含有微生物菌群的胶囊,并分析其特性,以提出最佳应用条件。TGA和DSC结果表明胶囊在25-40°C范围内具有热稳定性。在含有 1% (w/v) 海藻酸钠的胶囊中,活细胞计数更有效,乳化指数从第 5 天起大幅增加(80%),表面张力在同一时期。乳化指数的增加是由细菌联合体产生的生物表面活性剂(两亲分子)的增加引起的,这提供了微生物与油之间更大的接触,为油的降解提供了最佳条件。因此,所有分析都显示了未来在石油泄漏中应用的出色结果。乳化指数较第5天有较大增加(80%),而表面张力则较同期有较大下降(48%)。乳化指数的增加是由细菌聚生体产生的生物表面活性剂(两亲分子)的增加引起的,这提供了微生物与油之间更大的接触,为油的降解提供了最佳条件。因此,所有分析都显示了未来在石油泄漏中应用的出色结果。乳化指数较第5天有较大增加(80%),而表面张力则较同期有较大下降(48%)。乳化指数的增加是由细菌聚生体产生的生物表面活性剂(两亲分子)的增加引起的,这提供了微生物与油之间更大的接触,为油的降解提供了最佳条件。因此,所有分析都显示了未来在石油泄漏中应用的出色结果。乳化指数的增加是由细菌聚生体产生的生物表面活性剂(两亲分子)的增加引起的,这提供了微生物与油之间更大的接触,为油的降解提供了最佳条件。因此,所有分析都显示了未来在石油泄漏中应用的出色结果。乳化指数的增加是由细菌聚生体产生的生物表面活性剂(两亲分子)的增加引起的,这提供了微生物与油之间更大的接触,为油的降解提供了最佳条件。因此,所有分析都显示了未来在石油泄漏中应用的出色结果。

更新日期:2021-07-09
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